LOCUS       sequence-154150-         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSB3K3 carrying J109-30hrpR-32hrpS-t-hrpL-30gfp-t.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Wang B, Barahona M, Buck M
  TITLE     Engineering modular and tunable genetic amplifiers for scaling 
            transcriptional signals in cascaded gene networks.
  JOURNAL   Nucleic Acids Res. 2014 Aug;42(14):9484-92. doi: 10.1093/nar/gku593.
            Epub 2014 Jul 16.
  PUBMED    25030903
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku593"; journalName: "Nucleic Acids Res"; date: 
            "2014-08"; volume: "42"; issue: "14"; pages: "9484-92"
COMMENT     Sequence Label: pBW211J109-Amp32C
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..21
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(25..68)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    71..92
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     RBS             137..148
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg
       61 atgatttctg gaattcgcgg ccgcttctag agtttacagc tagctcagtc ctagggactg
      121 tgctactcta gagattaaag aggagaaata ctagatgagt acaggcatcg ataaggacgt
      181 ccgagagtgt tggggcgtaa ctgcattatc agcgggtcat caaattgcaa tgaatagcgc
      241 gtttctggat atggacttgc tgttgtgcgg ggaaaccggc accggcaagg acacactggc
      301 caaccgcatt cacgagttgt ccagcaggtc gggacccttt gtgggcatga actgcgccgc
      361 cattcccgag tcgctggcag agagccagtt attcggtgtg gtcaacggtg cattcaccgg
      421 cgtatgccgg gctcgcgagg gctacataga ggcctccagt ggtggcacct tgtacctgga
      481 tgaaatcgac agcatgccgt tgagcctgca agccaaactg ctgcgtgtgt tggagagtcg
      541 aggtatcgag cgtctgggct cgaccgaatt tatcccggtg gatctgcgga tcattgcctc
      601 ggcccagcgg ccactggatg aactggtgga acaaggactt ttccgtcgcg acctgttttt
      661 tcggctcaac gtgctgacgc ttcacttgcc agccttgcgc aaacgtcgtg aacagatcct
      721 gccattgttc gaccagttca cccagggtat cgctgccgag ttcggacgtc ccgctcctgc
      781 gctggacagc gggcgtgtgc agctgctgct cagccacgac tggccgggca acatccgcga
      841 a
//
