LOCUS       sequence-154153-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSB3K3 carrying J114-30hrpR-32hrpS-t-hrpL-30gfp-t.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Wang B, Barahona M, Buck M
  TITLE     Engineering modular and tunable genetic amplifiers for scaling 
            transcriptional signals in cascaded gene networks.
  JOURNAL   Nucleic Acids Res. 2014 Aug;42(14):9484-92. doi: 10.1093/nar/gku593.
            Epub 2014 Jul 16.
  PUBMED    25030903
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku593"; journalName: "Nucleic Acids Res"; date: 
            "2014-08"; volume: "42"; issue: "14"; pages: "9484-92"
COMMENT     Sequence Label: pBW212J114-Amp32C
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..21
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(25..68)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    71..92
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        93..127
                     /label=BBa_J23114
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.1"
     RBS             139..150
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg
       61 atgatttctg gaattcgcgg ccgcttctag agtttatggc tagctcagtc ctaggtacaa
      121 tgctagctac tagagattaa agaggagaaa tactagatga gtacaggcat cgataaggac
      181 gtccgagagt gttggggcgt aactgcatta tcagcgggtc atcaaattgc aatgaatagc
      241 gcgtttctgg atatggactt gctgttgtgc ggggaaaccg gcaccggcaa ggacacactg
      301 gccaaccgca ttcacgagtt gtccagcagg tcgggaccct ttgtgggcat gaactgcgcc
      361 gccattcccg agtcgctggc agagagccag ttattcggtg tggtcaacgg tgcattcacc
      421 ggcgtatgcc gggctcgcga gggctacata gaggcctcca gtggtggcac cttgtacctg
      481 gatgaaatcg acagcatgcc gttgagcctg caagccaaac tgctgcgtgt gttggagagt
      541 cgaggtatcg agcgtctggg ctcgaccgaa tttatcccgg tggatctgcg gatcattgcc
      601 tcggcccagc ggccactgga tgaactggtg gaacaaggac ttttccgtcg cgacctgttt
      661 tttcggctca acgtgctgac gcttcacttg ccagccttgc gcaaacgtcg tgaacagatc
      721 ctgccattgt tcgaccagtt cacccagggt atcgctgccg agttcggacg tcccgctcct
      781 gcgctggaca gcgggcgtgt gcagctgctg ctcagccacg actggccggg caacatccgc
      841 gaattgaagt ctgcggccaa gcgcttcgta ctcggcttcc cc
//
