LOCUS       sequence-154157-         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSB3K3 carrying J116-30hrpR-32hrpS-t-hrpL-30gfp-t.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Wang B, Barahona M, Buck M
  TITLE     Engineering modular and tunable genetic amplifiers for scaling 
            transcriptional signals in cascaded gene networks.
  JOURNAL   Nucleic Acids Res. 2014 Aug;42(14):9484-92. doi: 10.1093/nar/gku593.
            Epub 2014 Jul 16.
  PUBMED    25030903
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku593"; journalName: "Nucleic Acids Res"; date: 
            "2014-08"; volume: "42"; issue: "14"; pages: "9484-92"
COMMENT     Sequence Label: pBW213J116-Amp32C
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..20
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(24..67)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    70..91
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        92..126
                     /label=BBa_J23116
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.16"
     RBS             138..149
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt tcgctaagga
       61 tgatttctgg aattcgcggc cgcttctaga gttgacagct agctcagtcc tagggactat
      121 gctagctact agagattaaa gaggagaaat actagatgag tacaggcatc gataaggacg
      181 tccgagagtg ttggggcgta actgcattat cagcgggtca tcaaattgca atgaatagcg
      241 cgtttctgga tatggacttg ctgttgtgcg gggaaaccgg caccggcaag gacacactgg
      301 ccaaccgcat tcacgagttg tccagcaggt cgggaccctt tgtgggcatg aactgcgccg
      361 ccattcccga gtcgctggca gagagccagt tattcggtgt ggtcaacggt gcattcaccg
      421 gcgtatgccg ggctcgcgag ggctacatag aggcctccag tggtggcacc ttgtacctgg
      481 atgaaatcga cagcatgccg ttgagcctgc aagccaaact gctgcgtgtg ttggagagtc
      541 gaggtatcga gcgtctgggc tcgaccgaat ttatcccggt ggatctgcgg atcattgcct
      601 cggcccagcg gccactggat gaactggtgg aacaaggact tttccgtcgc gacctgtttt
      661 ttcggctcaa cgtgctgacg cttcacttgc cagccttgcg caaacgtcgt gaacagatcc
      721 tgccattgtt cgaccagttc acccagggta tcgctgccga gttcggacgt cccgctcctg
      781 cgctggacag tgggcgtgtg cagctgctgc tcagccacga ctggccgggc aacatccgcg
      841 aattgaagtc tgcggccaag cgcttcgtac tcgg
//
