LOCUS       sequence-154163-        1017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a catalytically inactive form of human PMR1 endonuclease 
            with N-terminal FLAG and C-terminal biotinylation tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Gu SQ, Bakthavachalu B, Han J, Patil DP, Otsuka Y, Guda C, 
            Schoenberg DR
  TITLE     Identification of the human PMR1 mRNA endonuclease as an 
            alternatively processed product of the gene for peroxidasin-like 
            protein.
  JOURNAL   RNA. 2012 Jun;18(6):1186-96. doi: 10.1261/rna.031369.111. Epub 2012 
            Apr 27.
  PUBMED    22543864
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.031369.111"; journalName: "RNA"; date: "2012-06"; 
            volume: "18"; issue: "6"; pages: "1186-96"
COMMENT     Sequence Label: pcDNA4/TO/FLAG-hPMR1(2H)-Tev-Bio
FEATURES             Location/Qualifiers
     source          1..1017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    4..22
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    25..43
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     53..77
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             177..200
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      714..723
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctctccctat cagtgataga gatctcccta tcagtgatag agatcgtcga cgagctcgtt
       61 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
      121 accgggaccg atccagcctc cggactctag cgtttaaact taagcttggt accatggact
      181 acaaggacga cgatgacaag atggccatgg aggccatgct catgcactgg ggctggtttc
      241 tagagcacga cttggaccac acagtgcctg cgctgagcac agcccgcttc tcggatgggc
      301 ggccgtgcag ctccgtctgc accaacgacc ctccttgttt ccccatgaac acccggcacg
      361 ccgacccccg gggcacccac gcgccctgca tgctcttcgc gcgctccagc cccgcgtgtg
      421 ccagcggccg tccctctgcg acggtggatt cagtctatgc acgagagcag atcaaccagc
      481 aaacagccta catcgatggc tccaacgttt acgggagctc ggagcgggaa tcccaggctc
      541 tcagagaccc ttcggtgcct cggggtctcc tgaagacagg ctttccttgg cctccctccg
      601 gaaagccctt attgcccttt tctacaggcc cacccaccga gtgcgcgcga caggagcagg
      661 agagcccctg tttcctggcc ggggaccacc gggccaacga gcatctggct ctggccgcca
      721 tggctaccct gtggttccgg gaacacaaca gggtggccac ggagctgtcc gccctgaacc
      781 cccactggga gggaaacacg gtttaccagg aagccaggaa gatcgtgggc gcggagctgc
      841 agcacatcac ctacagccac tggctgccta aggtcctggg ggaccctggc actaggatgc
      901 tgaggggtta ccgaggctac aaccccaacg tgaatgcagg catcattaac tcttttgcta
      961 ctgcagcctt tagatttggc gccacattaa tcaatcctat tctttaccga ctgaatg
//
