LOCUS       sequence-154168-         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSB3K3 carrying J105-30hrpR-32hrpS-t-hrpL-30gfp-t.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Wang B, Barahona M, Buck M
  TITLE     Engineering modular and tunable genetic amplifiers for scaling 
            transcriptional signals in cascaded gene networks.
  JOURNAL   Nucleic Acids Res. 2014 Aug;42(14):9484-92. doi: 10.1093/nar/gku593.
            Epub 2014 Jul 16.
  PUBMED    25030903
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku593"; journalName: "Nucleic Acids Res"; date: 
            "2014-08"; volume: "42"; issue: "14"; pages: "9484-92"
COMMENT     Sequence Label: pBW215J105-Amp32C
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             51..62
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(296..323)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(339..410)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 agaattggga caactccagt gaaaagttct tctcctttac gcatctagta tttctcctct
       61 ttaatctcta gtagtacaag ccctatagcg ataaccatgc cagcttaaaa atagaaataa
      121 ctgattgatt tataaagata ttttaaaatg tgaaattctt tgtaatacaa aactttgcgc
      181 aaaccgtttt ttgccacaac agcttttggc acaaaaacat cggtcagctt cgcaagggaa
      241 ctgatccggg accgtgaccc actcagcgga cataatccgg cctctagtat ataaacgcag
      301 aaaggcccac ccgaaggtga gccagtgtga ctctagtaga gagcgttcac cgacaaacaa
      361 cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctggtt
      421 attagatctg caattctttg atgcgtcgat agagcgtacg gcgtggcacg tccagttcca
      481 ggcttaccga atccacacaa ttgtcgtggc gcttgagcga ctcctgaatc agggcttttt
      541 cgatcactcg caattgcgat ttgagcccac aggccaagtg ctcttcgccc tgcggctcgg
      601 cgcccagcaa ggggaaaccc agcacatggc gtttggctgc cgccttgagc tcacggatat
      661 tgccgggcca gtcgtggccc agcagcactt tgtgcagcag tgggcaaaca tcgggaacgg
      721 gaacaccgag ctccctcgcg gcggcggccg taaaacgtgt g
//
