LOCUS       sequence-154176-         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSB3K3 carrying J106-30hrpR-32hrpS-t-hrpL-30gfp-tT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Wang B, Barahona M, Buck M
  TITLE     Engineering modular and tunable genetic amplifiers for scaling 
            transcriptional signals in cascaded gene networks.
  JOURNAL   Nucleic Acids Res. 2014 Aug;42(14):9484-92. doi: 10.1093/nar/gku593.
            Epub 2014 Jul 16.
  PUBMED    25030903
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku593"; journalName: "Nucleic Acids Res"; date: 
            "2014-08"; volume: "42"; issue: "14"; pages: "9484-92"
COMMENT     Sequence Label: pBW216J106-Amp32C
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             39..50
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(284..311)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(327..398)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 actccagtga aaagttcttc tcctttacgc atctagtatt tctcctcttt aatctctagt
       61 agtacaagcc ctatagcgat aaccatgcca gcttaaaaat agaaataact gattgattta
      121 taaagatatt ttaaaatgtg aaattctttg taatacaaaa ctttgcgcaa accgtttttt
      181 gccacaacag cttttggcac aaaaacatcg gtcagcttcg caagggaact gatccgggac
      241 cgtgacccac tcagcggaca taatccggcc tctagtatat aaacgcagaa aggcccaccc
      301 gaaggtgagc cagtgtgact ctagtagaga gcgttcaccg acaaacaaca gataaaacga
      361 aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctggttat tagatctgca
      421 attctttgat gcgtcgatag agcgtacggc gtggcacgtc cagttccagg cttaccgaat
      481 ccacacaatt gtcgtggcgc ttgagcgact cctgaatcag ggctttttcg atcactcgca
      541 attgcgattt gagcccacag gccaagtgct cttcgccctg cggctcggcg cccagcaagg
      601 ggaaacccag cacatggcgt ttggctgccg ccttgagctc acggatattg ccgggccagt
      661 cgtggcccag cagcactttg tgcagcagtg ggcaaacatc gggaacggga acaccgagct
      721 ccctcgcggc ggcggccgta aaacgtgtga acaggggaac tatgcgatca gactggttac
      781 gtagcggagg aagcttgagt gtcaggacgt tcaggcgaaa atacagatcg cgacgaaact
      841 gcccccgctc gacggcgtcg tccagcgagc attggg
//
