LOCUS       sequence-154179-        1083 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express and target GFP into the Golgi apparatus using 
            lentiviruses.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1083)
  TITLE     pLV-mGFP Golgi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-Golgi eGFP
FEATURES             Location/Qualifiers
     source          1..1083
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      339..348
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(345..347,348..350,351..1064)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(390..411)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(651..670)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     998..1019
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagac accgcccggg cgtacgggat cccaccggtg ctcgagacta
      121 gtatgaggct tcgggagccg ctcctgagcg gcagcgccgc gatgccaggc gcgtccctac
      181 agcgggcctg ccgcctgctc gtggccgtct gcgctctgca ccttggcgtc accctcgttt
      241 actacctggc tggccgcgac ctgagccgcc tgccccaact ggtcggagtc tccacaccgc
      301 tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg agcaagggcg
      361 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
      421 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
      481 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
      541 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
      601 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
      661 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
      721 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
      781 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
      841 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
      901 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
      961 ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     1021 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaatctaga cctgcagggt
     1081 cga
//
