LOCUS       sequence-154184-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSB3K3 carrying 
            araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-30hrpR-32hrpS-t-hrpL-30gfp-t.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Wang B, Barahona M, Buck M
  TITLE     Engineering modular and tunable genetic amplifiers for scaling 
            transcriptional signals in cascaded gene networks.
  JOURNAL   Nucleic Acids Res. 2014 Aug;42(14):9484-92. doi: 10.1093/nar/gku593.
            Epub 2014 Jul 16.
  PUBMED    25030903
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku593"; journalName: "Nucleic Acids Res"; date: 
            "2014-08"; volume: "42"; issue: "14"; pages: "9484-92"
COMMENT     Sequence Label: pBW300ParsR-Amp32T
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             29..40
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             179..190
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      418..489
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      505..532
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        547..581
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
ORIGIN
        1 actctctact gtttctccat tactagagaa agaggagaaa tactagatga ttgaggtaac
       61 ggaaaagtcg gcattttacg ctcaggttgc cgcccagagc ccagcagtat ggccagtggc
      121 caatggcgtc gcgtttgtca gtcggcgcga acaccatgat tgggggatcg ccttgcacat
      181 agaaggccgc gcgttgcgtc ccgatcaact gcgtgatgca ctgcaacgac gttttatgga
      241 gtcagagcgt ttcaatcact acttcctgtt tctggacgta cgacgcgact ttgtggtgtg
      301 gcacgcggtc aacgaaaaac cgggttccta cgccagcctg gacgacatcc gccggcatga
      361 gttgatgctg gcagggctgg accatttgag cgaggaaatg cactaataac caggcatcaa
      421 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
      481 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata
      541 ctagagttta cagctagctc agtccttggt attatgctag ctcctagggt cacacaggaa
      601 agtaccatat gtcatttctg ttacccatcc aattgttcaa aattcttgct gatgaaaccc
      661 gtctgggcat cgttttactg ctcagcgaac tgggagagtt atgcgtctgc gatctctgca
      721 ctgctctcga ccagtcgcag cccaagatct cccgccacct ggcattgctg cgtgaaagcg
      781 ggctattgct ggaccgcaag caaggtaagt gggttcatta ccgcttatca ccgcatatt
//
