LOCUS       sequence-154191-        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express and target GFP into the endoplasmic reticulum using 
            lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  TITLE     pLV-ER eGFP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-ER GFP
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             117..833
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagac accgcccggg cgtacgggat cccaccggtg ctcgagatgg
      121 tgagcaaggg cgccgagctg ttcaccggca tcgtgcccat cctgatcgag ctgaatggcg
      181 atgtgaatgg ccacaagttc agcgtgagcg gcgagggcga gggcgatgcc acctacggca
      241 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc tgtgccctgg cccaccctgg
      301 tgaccaccct gagctacggc gtgcagtgct tctcacgcta ccccgatcac atgaagcagc
      361 acgacttctt caagagcgcc atgcctgagg gctacatcca ggagcgcacc atcttcttcg
      421 aggatgacgg caactacaag tcgcgcgccg aggtgaagtt cgagggcgat accctggtga
      481 atcgcatcga gctgaccggc accgatttca aggaggatgg caacatcctg ggcaataaga
      541 tggagtacaa ctacaacgcc cacaatgtgt acatcatgac cgacaaggcc aagaatggca
      601 tcaaggtgaa cttcaagatc cgccacaaca tcgaggatgg cagcgtgcag ctggccgacc
      661 actaccagca gaataccccc atcggcgatg gccctgtgct gctgcccgat aaccactacc
      721 tgtccaccca gagcgccctg tccaaggacc ccaacgagaa gcgcgatcac atgatctact
      781 tcggcttcgt gaccgccgcc gccatcaccc acggcatgga tgagctgtac aagtccggac
      841 tcagatctat gcctggtccg acccccagtg gcactaacgt gggatcctca gggcgctctc
      901 ccagcaaagc agtggccgcc cgggcggcgg gatccactgt ccggcagagg aaaaatgcca
      961 gctgtgggac aaggagtgca ggccgcacaa cctcggcagg caccgggggg
//
