LOCUS       sequence-154193-        1026 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express and target GFP into the endoplasmic reticulum using 
            lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1026)
  TITLE     pLV-ER eGFP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-ER GFP
FEATURES             Location/Qualifiers
     source          1..1026
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(116..406)
                     /codon_start=1
                     /gene="SEC61B"
                     /product="human Sec61-beta subunit of the translocon in the
                     endoplasmic reticulum"
                     /label=Sec61-beta
                     /translation="MPGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAG
                     RTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS"
ORIGIN
        1 ggagcaacat agttaagaat accagtcaat ctttcacaaa ttttgtaatc cagaggttga
       61 ttgtcgatat ctgtacaggg ccctgatcat ccggagtcga ccctgcaggt ctagactacg
      121 aacgagtgta cttgccccaa atgtgcaaca taaatacaga agcgatgaac agaagactca
      181 taaccaatac tggaacaggg ccaactttga gcccaggtga atcttctgtg tagaatcgcc
      241 acatcccccc ggtgcctgcc gaggttgtgc ggcctgcact ccttgtccca cagctggcat
      301 ttttcctctg ccggacagtg gatcccgccg cccgggcggc cactgctttg ctgggagagc
      361 gccctgagga tcccacgtta gtgccactgg gggtcggacc aggcatagat ctgagtccgg
      421 acttgtacag ctcatccatg ccgtgggtga tggcggcggc ggtcacgaag ccgaagtaga
      481 tcatgtgatc gcgcttctcg ttggggtcct tggacagggc gctctgggtg gacaggtagt
      541 ggttatcggg cagcagcaca gggccatcgc cgatgggggt attctgctgg tagtggtcgg
      601 ccagctgcac gctgccatcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccat
      661 tcttggcctt gtcggtcatg atgtacacat tgtgggcgtt gtagttgtac tccatcttat
      721 tgcccaggat gttgccatcc tccttgaaat cggtgccggt cagctcgatg cgattcacca
      781 gggtatcgcc ctcgaacttc acctcggcgc gcgacttgta gttgccgtca tcctcgaaga
      841 agatggtgcg ctcctggatg tagccctcag gcatggcgct cttgaagaag tcgtgctgct
      901 tcatgtgatc ggggtagcgt gagaagcact gcacgccgta gctcagggtg gtcaccaggg
      961 tgggccaggg cacaggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt
     1021 aggtgg
//
