LOCUS       sequence-154204-        1079 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pET-Duet for bacterial expression of Cys-NuG2(4x)-Ald and 
            co-expression of Mycobacterium tuberculosis Formylglycine Generating
            Enzyme.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Walder R, LeBlanc MA, Van Patten WJ, Edwards D, Greenberg JA, 
            Adhikari A, Okoniewski SR, Sullan RMA, Rabuka D, Sousa MC, Perkins 
            TT
  TITLE     Rapid Characterization of a Mechanically Labile alpha-helical 
            Protein Enabled by Efficient Site-Specific Bioconjugation.
  JOURNAL   J Am Chem Soc. 2017 Jul 5. doi: 10.1021/jacs.7b02958.
  PUBMED    28677396
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2017 Jul 5. doi: 10.1021/jacs.7b02958."
COMMENT     Sequence Label: pMS1088
FEATURES             Location/Qualifiers
     source          1..1079
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttaagcatta tgcggccgca agcttgtcga cctgcaggcg cgccgagctc tcaagaccgc
       61 gatggtgtgc acagtccaga cgagcgttcg gtcacggtaa aggtcttggt ggcgtcggcg
      121 taggtccatt cgccgtccac accattatcg ttggcatatt gcttgaagac tttttctgcg
      181 gttgctgcat caacggcttc ggtcgtatac gtaaaggtgg tgccattcag gacgataacc
      241 agtttatagg tgtccatgct acgttccgtc accgtgaacg ttttggtggc gtctgcgtag
      301 gtccactcgc catccacacc attatcgttt gcatactgct tgaacacttt ttcagccgta
      361 gctgcatcga ctgcttccgt ggtgtacgtg aaggtggtgc cattcagcac aatgaccagt
      421 ttatacgtgt ccatggatcc ccgcgggata tcgccgctac gttccgtaac ggtaaacgtt
      481 ttggtggcat ccgcgtaggt ccattcacca tcgacaccat tatcgtttgc gtattgcttg
      541 aaaacctttt ctgccgttgc tgcatccact gcttccgtgg tgtaggtaaa cgtggtgccg
      601 ttcagaacga taaccagctt gtacgtgtcc atactacgtt ccgtcacggt gaacgttttg
      661 gtagcatctg cgtaggtcca ttcgccgtca acaccattat cgttcgcata ctgcttaaac
      721 actttttccg ccgtcgccgc atcaactgct tccgtcgtgt aggtaaaggt cgtgccattc
      781 agaacaatca ccagcttata ggtatccatt ccagagcagc cgccaatctg ttctctgtga
      841 gcctcaataa tatcgttatc ctccatgtcc aaatcttcag gggtctgatc agcttgaatt
      901 ctaataccgt cgtacaagaa tcttaaggag tccatttcct taccctgtct tttagcgaac
      961 gcttccatca gccttcttaa aggagtggtc tttttgatct tgaagaagat ctctgaagat
     1021 ccatcggaca cctttaaatt gatgtgagtc tcaggcttga cttctggctt gacctctgg
//
