LOCUS       sequence-154238-         272 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Single Integration Vector HIS - Cloning vector for single 
            integration into the HIS3 locus of S.cerevisiae containing two MCS..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: SIVh
FEATURES             Location/Qualifiers
     source          1..272
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    219..252
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     253..272
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        253..271
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gacaataaaa agattcttgt tttcaagaac ttgtcatttg tatagttttt ttatattgta
       61 gttgttctat tttaatcaaa tgttagcgtg atttatattt tttttcgcct cgacatcatc
      121 tgcccagatg cgaagttaag tgcgcagaaa gtaatatcat gcgtcaatcg tatgtgaatg
      181 ctggtcgcta tactgctgtc gattcgatac taacgccgat aacttcgtat aatgtatgct
      241 atacgaagtt attaatacga ctcactatag gg
//
