LOCUS       sequence-154244-        4294 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene Tagging vector URA/CFP - Plasmid for yeast enhanced monomeric 
            CFP (yemCFP) labeling of endogenous proteins with a URA selection 
            marker, originating from pSIVu (ID:81089).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTU-c
FEATURES             Location/Qualifiers
     source          1..4294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(31..94)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        121..139
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             171..887
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYIYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSRLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      931..1118
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(986..1010)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        1193..1536
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      2355..2552
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    2576..2609
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(2654..2672)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2705..3293)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2785..2804)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3464..4255,4256..4294,1..30))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4087..4106
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
       61 ttatcagggt tattgtctca tgagcggata catatttgaa gtatcgttcg aattaattaa
      121 aattaaccct cactaaaggg gcatgcgcgg ccgctctaga actaattaac atgtctaaag
      181 gtgaagaatt attcactggt gttgtcccaa ttttggttga attagatggt gatgttaatg
      241 gtcacaaatt ttctgtctcc ggtgaaggtg aaggtgatgc tacttacggt aaattgacct
      301 taaaatttat ttgtactact ggtaaattgc cagttccatg gccaacctta gtcactactt
      361 taacttgggg tgttcaatgt ttttctagat acccagatca tatgaaacaa catgactttt
      421 tcaagtctgc catgccagaa ggttatgttc aagaaagaac tatttttttc aaagatgacg
      481 gtaactacaa gaccagagct gaagtcaagt ttgaaggtga taccttagtt aatagaatcg
      541 aattaaaagg tattgatttt aaagaagatg gtaacatttt aggtcacaaa ttggaataca
      601 tttataactc tcacaatgtt tacatcactg ctgacaaaca aaagaatggt atcaaagcta
      661 acttcaaaat tagacacaac attgaagatg gttctgttca attagctgac cattatcaac
      721 aaaatactcc aattggtgat ggtccagtct tgttaccaga caaccattac ttatccactc
      781 aatctagatt atccaaagat ccaaacgaaa agagagacca catggtcttg ttagaatttg
      841 ttactgctgc tggtattacc catggtatgg atgaattgta caaataagga tgctagcatt
      901 ttaactcgag ggcgcgccac ttctaaataa gcgaatttct tatgatttat gatttttatt
      961 attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta
     1021 aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta
     1081 tagtatgagg tcgctcttat tgaccacacc tctaccggca gatccgctag ggataacagg
     1141 gtaatataga tctgtttagc ttgcctcgtc cccgccgggt cacccggcga gcgacatgga
     1201 ggcccagaat accctccttg acagtcttga cgtgcgcagc tcaggggcat gatgtgactg
     1261 tcgcccgtac atttagccca tacatcccca tgtataatca tttgcatcca tacattttga
     1321 tggccgcacg gcgcgaagca aaaattacgg ctcctcgcct cagacctgcg agcagggaaa
     1381 cgctcccctc acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga gaaatataaa
     1441 aggttaggat ttgccactga ggttcttctt tcatatactt ccttttaaaa tcttgctagg
     1501 atacagttct cacatcacat ccgaacataa acaaccatga cagtcaacac taagacctat
     1561 agtgagagag cagaaactca tgcctcacca gtagcacagc gattatttcg attaatggaa
     1621 ctgaagaaaa ccaatttatg tgcatcaatt gacgttgata ccactaagga gttccttgaa
     1681 ttaattgata aattaggtcc ttatgtatgc ttaatcaaga ctcatattga tataatcaat
     1741 gatttttcct atgaatccac tattgaacca ttattagaac tttcacgtaa acatcaattt
     1801 atgatttttg aagatagaaa atttgctgat attggtaata ccgtaaagaa acaatatatt
     1861 ggtggagttt ataaaattag tagttgggca gatattacca atgctcatgg tgtcactggg
     1921 aatggagtgg ttgaaggatt aaaacaggga gctaaagaaa ccaccaccaa ccaagagcca
     1981 agagggttat tgatgttagc tgaattatca tcagtgggat cattagcata cggagaatat
     2041 tctcaaaaaa ctgttgaaat tgctaaatcc gataaggaat ttgttattgg atttattgcc
     2101 caacgtgata tgggtggcca agaagaagga tttgattggc ttattatgac acctggagtt
     2161 ggattagatg ataaaggtga tggattagga caacaatata gaactgttga tgaagttgtt
     2221 agcactggaa ctgatattat cattgttggt agaggattgt ttggtaaagg aagagatcca
     2281 gatattgaag gtaaaaggta tagaaatgct ggttggaatg cttatttgaa aaagactggc
     2341 caattataat cagtactgac aataaaaaga ttcttgtttt caagaacttg tcatttgtat
     2401 agttttttta tattgtagtt gttctatttt aatcaaatgt tagcgtgatt tatatttttt
     2461 ttcgcctcga catcatctgc ccagatgcga agttaagtgc gcagaaagta atatcatgcg
     2521 tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat tcgatactaa cgccgataac
     2581 ttcgtataat gtatgctata cgaagttatt aatacgactc actatacgaa ttggagctcc
     2641 aattcggacg tcccctatag tgagtcgtat tattaattaa ttcgaaggcc gcgttgctgg
     2701 cgtttttcca taggctcggc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     2761 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     2821 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     2881 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     2941 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     3001 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     3061 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     3121 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     3181 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     3241 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     3301 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     3361 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     3421 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     3481 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactgcccg
     3541 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     3601 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     3661 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     3721 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     3781 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     3841 gatcaaggcg agttacatga tcccccatgt tgtgaaaaaa agcggttagc tccttcggtc
     3901 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     3961 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     4021 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     4081 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     4141 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     4201 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     4261 aaacaggaag gcaaaatgcc gcaaaaaagg gaat
//
