LOCUS       sequence-154254-        4289 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene Tagging vector URA/YFP - Plasmid for monomeric Citrine 
            (mCitrine) labeling of endogenous proteins with a URA selection 
            marker, originating from pSIVu (ID:81089).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTU-y
FEATURES             Location/Qualifiers
     source          1..4289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(67..130)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        157..175
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             207..917
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLKEF
                     VTAAGITHGMDELYK"
     terminator      962..1149
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1017..1041)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        1224..1567
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      2386..2583
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    2607..2640
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(2685..2703)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2736..3324)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2816..2835)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3495..4286,4287..4289,1..66))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4118..4137
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
       61 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
      121 cggatacata tttgaagtat cgttcgaatt aattaaaatt aaccctcact aaaggggcat
      181 gcgcggccgc tctagaacta tctgcttcta aaggtgaaga attattcact ggtgttgtcc
      241 caattttggt tgaattagat ggtgatgtta atggtcacaa attttctgtc tccggtgaag
      301 gtgaaggtga tgctacttac ggtaaattga ccttaaaatt tatttgtact actggtaaat
      361 tgccagttcc atggccaacc ttagtcacta ctttaggtta tggtttgatg tgttttgcta
      421 gatacccaga tcatatgaaa caacatgact ttttcaagtc tgccatgcca gaaggttatg
      481 ttcaagaaag aactattttt ttcaaagatg acggtaacta caagaccaga gctgaagtca
      541 agtttgaagg tgatacctta gttaatagaa tcgaattaaa aggtattgat tttaaagaag
      601 atggtaacat tttaggtcac aaattggaat acaactataa ctctcacaat gtttacatca
      661 tggctgacaa acaaaagaat ggtatcaaag ttaacttcaa aattagacac aacattgaag
      721 atggttctgt tcaattagct gaccattatc aacaaaatac tccaattggt gatggtccag
      781 tcttgttacc agacaaccat tacttatcct atcaatctaa attatccaaa gatccaaacg
      841 aaaagagaga ccacatggtc ttgaaagaat ttgttactgc tgctggtatt acccatggta
      901 tggatgaatt gtacaaagga atgctagcat tttaactcga gggcgcgcca cttctaaata
      961 agcgaatttc ttatgattta tgatttttat tattaaataa gttataaaaa aaataagtgt
     1021 atacaaattt taaagtgact cttaggtttt aaaacgaaaa ttcttattct tgagtaactc
     1081 tttcctgtag gtcaggttgc tttctcaggt atagtatgag gtcgctctta ttgaccacac
     1141 ctctaccggc agatccgcta gggataacag ggtaatatag atctgtttag cttgcctcgt
     1201 ccccgccggg tcacccggcg agcgacatgg aggcccagaa taccctcctt gacagtcttg
     1261 acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta catttagccc atacatcccc
     1321 atgtataatc atttgcatcc atacattttg atggccgcac ggcgcgaagc aaaaattacg
     1381 gctcctcgcc tcagacctgc gagcagggaa acgctcccct cacagacgcg ttgaattgtc
     1441 cccacgccgc gcccctgtag agaaatataa aaggttagga tttgccactg aggttcttct
     1501 ttcatatact tccttttaaa atcttgctag gatacagttc tcacatcaca tccgaacata
     1561 aacaaccatg acagtcaaca ctaagaccta tagtgagaga gcagaaactc atgcctcacc
     1621 agtagcacag cgattatttc gattaatgga actgaagaaa accaatttat gtgcatcaat
     1681 tgacgttgat accactaagg agttccttga attaattgat aaattaggtc cttatgtatg
     1741 cttaatcaag actcatattg atataatcaa tgatttttcc tatgaatcca ctattgaacc
     1801 attattagaa ctttcacgta aacatcaatt tatgattttt gaagatagaa aatttgctga
     1861 tattggtaat accgtaaaga aacaatatat tggtggagtt tataaaatta gtagttgggc
     1921 agatattacc aatgctcatg gtgtcactgg gaatggagtg gttgaaggat taaaacaggg
     1981 agctaaagaa accaccacca accaagagcc aagagggtta ttgatgttag ctgaattatc
     2041 atcagtggga tcattagcat acggagaata ttctcaaaaa actgttgaaa ttgctaaatc
     2101 cgataaggaa tttgttattg gatttattgc ccaacgtgat atgggtggcc aagaagaagg
     2161 atttgattgg cttattatga cacctggagt tggattagat gataaaggtg atggattagg
     2221 acaacaatat agaactgttg atgaagttgt tagcactgga actgatatta tcattgttgg
     2281 tagaggattg tttggtaaag gaagagatcc agatattgaa ggtaaaaggt atagaaatgc
     2341 tggttggaat gcttatttga aaaagactgg ccaattataa tcagtactga caataaaaag
     2401 attcttgttt tcaagaactt gtcatttgta tagttttttt atattgtagt tgttctattt
     2461 taatcaaatg ttagcgtgat ttatattttt tttcgcctcg acatcatctg cccagatgcg
     2521 aagttaagtg cgcagaaagt aatatcatgc gtcaatcgta tgtgaatgct ggtcgctata
     2581 ctgctgtcga ttcgatacta acgccgataa cttcgtataa tgtatgctat acgaagttat
     2641 taatacgact cactatacga attggagctc caattcggac gtcccctata gtgagtcgta
     2701 ttattaatta attcgaaggc cgcgttgctg gcgtttttcc ataggctcgg cccccctgac
     2761 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     2821 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     2881 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     2941 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     3001 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     3061 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     3121 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     3181 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     3241 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     3301 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     3361 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     3421 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     3481 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     3541 tttcgttcat ccatagttgc ctgactgccc gtcgtgtaga taactacgat acgggagggc
     3601 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     3661 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     3721 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     3781 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     3841 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     3901 ttgtgaaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     3961 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     4021 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     4081 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     4141 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     4201 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     4261 tttactttca ccagcgtttc tgggtgagc
//
