LOCUS       sequence-154264-        5738 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene Tagging vector URA/iRFP - Plasmid for tandem infrared RFP 
            (tdiRFP) labeling of endogenous proteins with a URA selection 
            marker, originating from pSIVu (ID:81089).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 5738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTU-ir
FEATURES             Location/Qualifiers
     source          1..5738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(31..94)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        121..139
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     179..195
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     180..196
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     224..240
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     224..240
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      2375..2562
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2430..2454)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        2637..2980
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      3799..3996
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    4020..4053
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(4098..4116)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(4149..4737)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4229..4248)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4908..5699,5700..5738,1..30))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5531..5550
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
       61 ttatcagggt tattgtctca tgagcggata catatttgaa gtatcgttcg aattaattaa
      121 aattaaccct cactaaaggg gcatgcaaca aaagctgggt acggtaccgg gccccccctc
      181 gaggtcgacg gtatcgataa gcttctcaag aactggcggc cgctctagaa ctagtggatc
      241 ctctagaact aatggcagag ggaagcgttg cccgtcaacc cgacctatta acttgcgatg
      301 atgaacctat ccatatcccc ggagcaattc aacctcacgg tctattgtta gcccttgctg
      361 ccgatatgac aatcgttgca ggttcagata acctaccgga attgaccggg ttagccattg
      421 gagccttgat cggccgttca gccgcagatg tgttcgattc agaaacacat aatagactga
      481 ccatcgcctt ggctgaacca ggggccgctg ttggcgctcc cattacagtt ggcttcacga
      541 tgagaaagga tgctggtttt attggatcat ggcatcgtca tgatcaactt attttcttgg
      601 aattggaacc tcctcaaagg gatgttgctg aacctcaggc tttctttcgt cgtactaata
      661 gcgctattcg tagacttcag gctgctgaaa cattggagag cgcttgcgca gcagcagctc
      721 aagaagttag aaagattacc ggttttgaca gagtaatgat atatagattt gcgtccgatt
      781 tctctggtga agttattgcg gaagatagat gtgccgaggt agagtcaaag ttggggttac
      841 attatcctgc ttcaacggtt ccagcacaag caaggcgtct gtacacaata aaccccgtaa
      901 gaataattcc agacatcaac tacagacctg tcccagtaac tcctgatctg aaccctgtga
      961 ctggtagacc tatagattta tcttttgcaa ttttgagatc agtgtcaccg gttcacttgg
     1021 agttcatgag aaatattggt atgcacggaa ccatgtccat ttctatactg aggggcgaaa
     1081 ggttgtgggg gctgattgtt tgccatcata ggacaccata ttatgtggac ttagacggta
     1141 ggcaggcgtg cgagctggtc gcccaagttt tggcttggca gattggtgtc atggaagaga
     1201 aggagggcca cggcaccggc agcaccggca gcggcagcag cggcagaagc ggcaccgcca
     1261 gcagcgagga caacaacatg gccagaatgc tagaactaat ggccgaaggt agcgttgcac
     1321 gtcaacccga tctactaaca tgcgatgatg aacctataca tattcctggg gctattcaac
     1381 ctcatggttt gttattggca ttggcagctg atatgacgat cgttgctgga agcgacaatt
     1441 tgccggagtt aactggacta gccatcggtg ctcttatagg tagatccgct gctgacgttt
     1501 tcgattcaga aacgcataac aggctaacga tcgctcttgc tgagcctggt gcagccgttg
     1561 gagcacccat caccgttggt tttactatga gaaaggatgc tggttttatt ggttcctggc
     1621 atagacatga ccaattaata ttcttagaat tggaacctcc ccagagggat gtcgcggaac
     1681 cacaagcatt ttttagaaga acaaattcag cgattagaag attacaagca gctgaaacac
     1741 ttgaatcagc atgtgcagca gctgcacagg aggtaaggaa gattacggga tttgacagag
     1801 taatgatcta tagatttgct agtgacttca gtggcgaagt tatagctgaa gatagatgtg
     1861 cggaggttga gagtaaactg ggtttacatt atccagcttc cacagttcca gcgcaagcaa
     1921 ggagattata cactataaac ccagtaagga tcatcccaga cattaactac agaccggtcc
     1981 cagtaactcc tgatttaaac ccagtaaccg gtagacctat tgacttgtct ttcgcaattt
     2041 taaggtctgt gtccccagtc catttggagt ttatgagaaa tatagggatg catgggacga
     2101 tgtcaatttc tatactgcgt ggtgagagac tgtggggact tattgtttgc catcatagaa
     2161 ctccatatta tgtggatctg gacggtagac aggcgtgtga acttgtggcc caggtgttag
     2221 cttggcagat cggagttatg gaagagaagg agggccacgg caccggcagc accggcagcg
     2281 gcagcagcgg cagaagcggc accgccagca gcgaggacaa caacatggcc agaatgctag
     2341 cattttaact cgagggcgcg ccacttctaa ataagcgaat ttcttatgat ttatgatttt
     2401 tattattaaa taagttataa aaaaaataag tgtatacaaa ttttaaagtg actcttaggt
     2461 tttaaaacga aaattcttat tcttgagtaa ctctttcctg taggtcaggt tgctttctca
     2521 ggtatagtat gaggtcgctc ttattgacca cacctctacc ggcagatccg ctagggataa
     2581 cagggtaata tagatctgtt tagcttgcct cgtccccgcc gggtcacccg gcgagcgaca
     2641 tggaggccca gaataccctc cttgacagtc ttgacgtgcg cagctcaggg gcatgatgtg
     2701 actgtcgccc gtacatttag cccatacatc cccatgtata atcatttgca tccatacatt
     2761 ttgatggccg cacggcgcga agcaaaaatt acggctcctc gcctcagacc tgcgagcagg
     2821 gaaacgctcc cctcacagac gcgttgaatt gtccccacgc cgcgcccctg tagagaaata
     2881 taaaaggtta ggatttgcca ctgaggttct tctttcatat acttcctttt aaaatcttgc
     2941 taggatacag ttctcacatc acatccgaac ataaacaacc atgacagtca acactaagac
     3001 ctatagtgag agagcagaaa ctcatgcctc accagtagca cagcgattat ttcgattaat
     3061 ggaactgaag aaaaccaatt tatgtgcatc aattgacgtt gataccacta aggagttcct
     3121 tgaattaatt gataaattag gtccttatgt atgcttaatc aagactcata ttgatataat
     3181 caatgatttt tcctatgaat ccactattga accattatta gaactttcac gtaaacatca
     3241 atttatgatt tttgaagata gaaaatttgc tgatattggt aataccgtaa agaaacaata
     3301 tattggtgga gtttataaaa ttagtagttg ggcagatatt accaatgctc atggtgtcac
     3361 tgggaatgga gtggttgaag gattaaaaca gggagctaaa gaaaccacca ccaaccaaga
     3421 gccaagaggg ttattgatgt tagctgaatt atcatcagtg ggatcattag catacggaga
     3481 atattctcaa aaaactgttg aaattgctaa atccgataag gaatttgtta ttggatttat
     3541 tgcccaacgt gatatgggtg gccaagaaga aggatttgat tggcttatta tgacacctgg
     3601 agttggatta gatgataaag gtgatggatt aggacaacaa tatagaactg ttgatgaagt
     3661 tgttagcact ggaactgata ttatcattgt tggtagagga ttgtttggta aaggaagaga
     3721 tccagatatt gaaggtaaaa ggtatagaaa tgctggttgg aatgcttatt tgaaaaagac
     3781 tggccaatta taatcagtac tgacaataaa aagattcttg ttttcaagaa cttgtcattt
     3841 gtatagtttt tttatattgt agttgttcta ttttaatcaa atgttagcgt gatttatatt
     3901 ttttttcgcc tcgacatcat ctgcccagat gcgaagttaa gtgcgcagaa agtaatatca
     3961 tgcgtcaatc gtatgtgaat gctggtcgct atactgctgt cgattcgata ctaacgccga
     4021 taacttcgta taatgtatgc tatacgaagt tattaatacg actcactata cgaattggag
     4081 ctccaattcg gacgtcccct atagtgagtc gtattattaa ttaattcgaa ggccgcgttg
     4141 ctggcgtttt tccataggct cggcccccct gacgagcatc acaaaaatcg acgctcaagt
     4201 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     4261 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     4321 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     4381 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     4441 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     4501 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     4561 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     4621 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     4681 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     4741 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     4801 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     4861 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     4921 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactg
     4981 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     5041 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     5101 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     5161 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     5221 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     5281 caacgatcaa ggcgagttac atgatccccc atgttgtgaa aaaaagcggt tagctccttc
     5341 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     5401 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     5461 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     5521 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     5581 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     5641 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     5701 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaat
//
