LOCUS       sequence-154274-        4760 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene Tagging vector LEU/GFP - Plasmid for superfolder GFP (sfGFP) 
            labeling of endogenous proteins with a LEU selection marker, 
            originating from pSIVl (ID:81090).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTL-g
FEATURES             Location/Qualifiers
     source          1..4760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(199..262)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        289..307
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      1085..1272
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1140..1164)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(1311..1327)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1311..1327)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    1328..1361
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    3207..3240
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3241..3259
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3288..3306)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3339..3927)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3419..3438)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4098..4760,1..129,130..198))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4721..4740
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
       61 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
      121 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
      181 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
      241 ttgtctcatg agcggataca tatttgaagt atcgttcgaa ttaattaaaa ttaaccctca
      301 ctaaaggggc atgcgcggcc gctctagaac tatccaaggg tgaagagcta tttactgggg
      361 ttgtacccat tttggtagaa ctggacggag atgtaaacgg acataaattc tctgttagag
      421 gtgagggcga aggcgatgcc accaatggta aattgactct gaagtttata tgcactacgg
      481 gtaaattacc tgttccttgg ccaaccctag taacaacttt gacatatggt gttcaatgtt
      541 tctcaagata cccagaccat atgaaaaggc atgatttctt taaaagtgct atgccagaag
      601 gctacgtgca agagagaact atctccttta aggatgacgg tacgtataaa acacgagcag
      661 aagtgaaatt cgaaggggat acactagtta atcgcatcga attaaagggt atagacttta
      721 aggaagatgg taatattctc ggccataaac ttgagtataa tttcaactcg cataatgtgt
      781 acattacagc tgacaaacaa aagaacggaa ttaaagcgaa ttttaaaatc aggcacaacg
      841 tcgaagatgg gtctgttcaa cttgccgatc attatcagca aaacacccct attggtgatg
      901 gtccagtctt gttacccgat aatcactact taagcacaca gtctagattg tcaaaagatc
      961 cgaatgaaaa gcgtgatcac atggttttat tggaatttgt caccgctgca ggaataactc
     1021 acggaatgga cgagctttat aagatgctag cattttaact cgagggcgcg ccacttctaa
     1081 ataagcgaat ttcttatgat ttatgatttt tattattaaa taagttataa aaaaaataag
     1141 tgtatacaaa ttttaaagtg actcttaggt tttaaaacga aaattcttat tcttgagtaa
     1201 ctctttcctg taggtcaggt tgctttctca ggtatagtat gaggtcgctc ttattgacca
     1261 cacctctacc ggcagatccg ctagggataa cagggtaata tagatccatg gtcatagctg
     1321 tttcctgata acttcgtata atgtatgcta tacgaagtta ttctgtaggc gaacctaacc
     1381 ggccaatttt ccaagaccgc tctgccgctg agcttgcacc cgtgccgcta cacaacaccc
     1441 acacatccgt aaaccccccg ccatcggtat caccaaggtt gtctatgacg aggcgtacaa
     1501 gaggacgtgg gggccgcctc tccaatgtca ccaataggaa aaaagaaaga ccgagtgcga
     1561 gtttctgaca gaagcctcaa gaattttttt tctttcttcg actatgctgg aggtagagat
     1621 gatcgaggca gtagtcaagt atatataaat taattagttc caccaccttc ttttcttgtg
     1681 ttgctccttt cgatgctatt tctggctttt tctaattttt ttcatcgttt cttactttct
     1741 tcctaatata taaattcttt tgcattttca ttcttcctct ccatttgttc attctcctta
     1801 aatttttttt aggtctcctt tctttcaaga aacacagtca tcgaactata attaactaaa
     1861 catgtcagca cctaagaaga tagtagtatt acctggagac cacgtaggac aggagataac
     1921 agcagaggca ataaaggtat taaaggcaat atcagacgta aggtcaaacg taaagttcga
     1981 cttcgagaac cacttaatag gaggagcagc aatagacgca acaggagtac ctttacctga
     2041 cgaggcatta gaggcatcaa agaaggcaga cgcagtatta ttaggagcag taggaggacc
     2101 taagtgggga acaggatcag taaggcctga gcagggatta ttaaagataa ggaaggagtt
     2161 acagttatac gcaaacttaa ggccttgcaa cttcgcatca gactcattat tagacttatc
     2221 acctataaag cctcagttcg caaagggaac agacttcgta gtagtaaggg agttagtagg
     2281 aggaatatac ttcggaaaga ggaaggagga cgacggagac ggagtagcat gggactcaga
     2341 gcagtacaca gtacctgagg tacagaggat aacaaggatg gcagcattca tggcattaca
     2401 gcacgagcct cctttaccta tatggtcatt agacaaggca aacgtattag catcatcaag
     2461 gttatggagg aagacagtag aggagacaat aaagaacgag ttccctacat taaaggtaca
     2521 gcaccagtta atagactcag cagcaatgat attagtaaag aaccctacac acttaaacgg
     2581 aataataata acatcaaaca tgttcggaga cataatatca gacgaggcat cagtaatacc
     2641 tggatcatta ggattattac cttcagcatc attagcatca ttacctgaca agaacacagc
     2701 attcggatta tacgagcctt gccacggatc agcacctgac ttacctaaga acaaggtaaa
     2761 ccctatagca acaatattat cagcagcaat gatgttaaag ttatcattaa acttacctga
     2821 ggagggaaag gcaatagagg acgcagtaaa gaaggtatta gacgcaggaa taaggacagg
     2881 agacttagga ggatcaaact caacaacaga ggtaggagac gcagtagcag aggaggtaaa
     2941 gaagatatta gcatgagaat attgataaat ttaaattttg gaatgagggt tgtcctttcc
     3001 tttacaatag aggcatttga gtgagtaggt tttatcacct tcttgctctt attaaatcgc
     3061 agttgtcttt ccggtatgtg cttgactgag gaacaatatt ttattcatga tacatttcca
     3121 tgctacgcat atcgtcatcc acatggaagg taattttctt ctgtggcctt tcatatagtt
     3181 gtgaatggta gaacaatctt catataataa cttcgtataa tgtatgctat acgaagttat
     3241 taatacgact cactataggg cgaattggag ctccaattcg gacgtcccct atagtgagtc
     3301 gtattattaa ttaattcgaa ggccgcgttg ctggcgtttt tccataggct cggcccccct
     3361 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     3421 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     3481 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     3541 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     3601 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     3661 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     3721 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     3781 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     3841 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     3901 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     3961 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     4021 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     4081 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     4141 ctatttcgtt catccatagt tgcctgactg cccgtcgtgt agataactac gatacgggag
     4201 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     4261 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     4321 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     4381 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     4441 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     4501 atgttgtgaa aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     4561 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     4621 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     4681 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     4741 agaactttaa aagtgctcat
//
