LOCUS       sequence-154279-        4769 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene Tagging vector LEU/YFP - Plasmid for monomeric Citrine 
            (mCitrine) labeling of endogenous proteins with a LEU selection 
            marker, originating from pSIVl (ID:81090).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTL-y
FEATURES             Location/Qualifiers
     source          1..4769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(88..151)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        178..196
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             228..938
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLKEF
                     VTAAGITHGMDELYK"
     terminator      983..1170
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1038..1062)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(1209..1225)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1209..1225)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    1226..1259
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    3105..3138
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3139..3157
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3186..3204)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3237..3825)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3317..3336)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3996..4769,1..18,19..87))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4619..4638
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
       61 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
      121 tcagggttat tgtctcatga gcggatacat atttgaagta tcgttcgaat taattaaaat
      181 taaccctcac taaaggggca tgcgcggccg ctctagaact atctgcttct aaaggtgaag
      241 aattattcac tggtgttgtc ccaattttgg ttgaattaga tggtgatgtt aatggtcaca
      301 aattttctgt ctccggtgaa ggtgaaggtg atgctactta cggtaaattg accttaaaat
      361 ttatttgtac tactggtaaa ttgccagttc catggccaac cttagtcact actttaggtt
      421 atggtttgat gtgttttgct agatacccag atcatatgaa acaacatgac tttttcaagt
      481 ctgccatgcc agaaggttat gttcaagaaa gaactatttt tttcaaagat gacggtaact
      541 acaagaccag agctgaagtc aagtttgaag gtgatacctt agttaataga atcgaattaa
      601 aaggtattga ttttaaagaa gatggtaaca ttttaggtca caaattggaa tacaactata
      661 actctcacaa tgtttacatc atggctgaca aacaaaagaa tggtatcaaa gttaacttca
      721 aaattagaca caacattgaa gatggttctg ttcaattagc tgaccattat caacaaaata
      781 ctccaattgg tgatggtcca gtcttgttac cagacaacca ttacttatcc tatcaatcta
      841 aattatccaa agatccaaac gaaaagagag accacatggt cttgaaagaa tttgttactg
      901 ctgctggtat tacccatggt atggatgaat tgtacaaagg aatgctagca ttttaactcg
      961 agggcgcgcc acttctaaat aagcgaattt cttatgattt atgattttta ttattaaata
     1021 agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa
     1081 attcttattc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg tatagtatga
     1141 ggtcgctctt attgaccaca cctctaccgg cagatccgct agggataaca gggtaatata
     1201 gatccatggt catagctgtt tcctgataac ttcgtataat gtatgctata cgaagttatt
     1261 ctgtaggcga acctaaccgg ccaattttcc aagaccgctc tgccgctgag cttgcacccg
     1321 tgccgctaca caacacccac acatccgtaa accccccgcc atcggtatca ccaaggttgt
     1381 ctatgacgag gcgtacaaga ggacgtgggg gccgcctctc caatgtcacc aataggaaaa
     1441 aagaaagacc gagtgcgagt ttctgacaga agcctcaaga attttttttc tttcttcgac
     1501 tatgctggag gtagagatga tcgaggcagt agtcaagtat atataaatta attagttcca
     1561 ccaccttctt ttcttgtgtt gctcctttcg atgctatttc tggctttttc taattttttt
     1621 catcgtttct tactttcttc ctaatatata aattcttttg cattttcatt cttcctctcc
     1681 atttgttcat tctccttaaa ttttttttag gtctcctttc tttcaagaaa cacagtcatc
     1741 gaactataat taactaaaca tgtcagcacc taagaagata gtagtattac ctggagacca
     1801 cgtaggacag gagataacag cagaggcaat aaaggtatta aaggcaatat cagacgtaag
     1861 gtcaaacgta aagttcgact tcgagaacca cttaatagga ggagcagcaa tagacgcaac
     1921 aggagtacct ttacctgacg aggcattaga ggcatcaaag aaggcagacg cagtattatt
     1981 aggagcagta ggaggaccta agtggggaac aggatcagta aggcctgagc agggattatt
     2041 aaagataagg aaggagttac agttatacgc aaacttaagg ccttgcaact tcgcatcaga
     2101 ctcattatta gacttatcac ctataaagcc tcagttcgca aagggaacag acttcgtagt
     2161 agtaagggag ttagtaggag gaatatactt cggaaagagg aaggaggacg acggagacgg
     2221 agtagcatgg gactcagagc agtacacagt acctgaggta cagaggataa caaggatggc
     2281 agcattcatg gcattacagc acgagcctcc tttacctata tggtcattag acaaggcaaa
     2341 cgtattagca tcatcaaggt tatggaggaa gacagtagag gagacaataa agaacgagtt
     2401 ccctacatta aaggtacagc accagttaat agactcagca gcaatgatat tagtaaagaa
     2461 ccctacacac ttaaacggaa taataataac atcaaacatg ttcggagaca taatatcaga
     2521 cgaggcatca gtaatacctg gatcattagg attattacct tcagcatcat tagcatcatt
     2581 acctgacaag aacacagcat tcggattata cgagccttgc cacggatcag cacctgactt
     2641 acctaagaac aaggtaaacc ctatagcaac aatattatca gcagcaatga tgttaaagtt
     2701 atcattaaac ttacctgagg agggaaaggc aatagaggac gcagtaaaga aggtattaga
     2761 cgcaggaata aggacaggag acttaggagg atcaaactca acaacagagg taggagacgc
     2821 agtagcagag gaggtaaaga agatattagc atgagaatat tgataaattt aaattttgga
     2881 atgagggttg tcctttcctt tacaatagag gcatttgagt gagtaggttt tatcaccttc
     2941 ttgctcttat taaatcgcag ttgtctttcc ggtatgtgct tgactgagga acaatatttt
     3001 attcatgata catttccatg ctacgcatat cgtcatccac atggaaggta attttcttct
     3061 gtggcctttc atatagttgt gaatggtaga acaatcttca tataataact tcgtataatg
     3121 tatgctatac gaagttatta atacgactca ctatagggcg aattggagct ccaattcgga
     3181 cgtcccctat agtgagtcgt attattaatt aattcgaagg ccgcgttgct ggcgtttttc
     3241 cataggctcg gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     3301 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     3361 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     3421 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     3481 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     3541 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     3601 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     3661 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     3721 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     3781 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     3841 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     3901 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     3961 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     4021 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactgcc cgtcgtgtag
     4081 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     4141 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     4201 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     4261 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     4321 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     4381 cgagttacat gatcccccat gttgtgaaaa aaagcggtta gctccttcgg tcctccgatc
     4441 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     4501 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     4561 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     4621 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     4681 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     4741 cccaactgat cttcagcatc ttttacttt
//
