LOCUS       sequence-154289-        6218 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene Tagging vector LEU/iRFP - Plasmid for tandem infrared RFP 
            (tdiRFP) labeling of endogenous proteins with a LEU selection 
            marker, originating from pSIVl (ID:81090).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 6218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTL-ir
FEATURES             Location/Qualifiers
     source          1..6218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(144..207)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        234..252
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     292..308
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     293..309
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     337..353
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     337..353
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      2488..2675
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2543..2567)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2714..2730)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2714..2730)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    2731..2764
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4610..4643
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4644..4662
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(4691..4709)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(4742..5330)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4822..4841)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5501..6218,1..74,75..143))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6124..6143
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
       61 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
      121 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
      181 ggttattgtc tcatgagcgg atacatattt gaagtatcgt tcgaattaat taaaattaac
      241 cctcactaaa ggggcatgca acaaaagctg ggtacggtac cgggcccccc ctcgaggtcg
      301 acggtatcga taagcttctc aagaactggc ggccgctcta gaactagtgg atcctctaga
      361 actaatggca gagggaagcg ttgcccgtca acccgaccta ttaacttgcg atgatgaacc
      421 tatccatatc cccggagcaa ttcaacctca cggtctattg ttagcccttg ctgccgatat
      481 gacaatcgtt gcaggttcag ataacctacc ggaattgacc gggttagcca ttggagcctt
      541 gatcggccgt tcagccgcag atgtgttcga ttcagaaaca cataatagac tgaccatcgc
      601 cttggctgaa ccaggggccg ctgttggcgc tcccattaca gttggcttca cgatgagaaa
      661 ggatgctggt tttattggat catggcatcg tcatgatcaa cttattttct tggaattgga
      721 acctcctcaa agggatgttg ctgaacctca ggctttcttt cgtcgtacta atagcgctat
      781 tcgtagactt caggctgctg aaacattgga gagcgcttgc gcagcagcag ctcaagaagt
      841 tagaaagatt accggttttg acagagtaat gatatataga tttgcgtccg atttctctgg
      901 tgaagttatt gcggaagata gatgtgccga ggtagagtca aagttggggt tacattatcc
      961 tgcttcaacg gttccagcac aagcaaggcg tctgtacaca ataaaccccg taagaataat
     1021 tccagacatc aactacagac ctgtcccagt aactcctgat ctgaaccctg tgactggtag
     1081 acctatagat ttatcttttg caattttgag atcagtgtca ccggttcact tggagttcat
     1141 gagaaatatt ggtatgcacg gaaccatgtc catttctata ctgaggggcg aaaggttgtg
     1201 ggggctgatt gtttgccatc ataggacacc atattatgtg gacttagacg gtaggcaggc
     1261 gtgcgagctg gtcgcccaag ttttggcttg gcagattggt gtcatggaag agaaggaggg
     1321 ccacggcacc ggcagcaccg gcagcggcag cagcggcaga agcggcaccg ccagcagcga
     1381 ggacaacaac atggccagaa tgctagaact aatggccgaa ggtagcgttg cacgtcaacc
     1441 cgatctacta acatgcgatg atgaacctat acatattcct ggggctattc aacctcatgg
     1501 tttgttattg gcattggcag ctgatatgac gatcgttgct ggaagcgaca atttgccgga
     1561 gttaactgga ctagccatcg gtgctcttat aggtagatcc gctgctgacg ttttcgattc
     1621 agaaacgcat aacaggctaa cgatcgctct tgctgagcct ggtgcagccg ttggagcacc
     1681 catcaccgtt ggttttacta tgagaaagga tgctggtttt attggttcct ggcatagaca
     1741 tgaccaatta atattcttag aattggaacc tccccagagg gatgtcgcgg aaccacaagc
     1801 attttttaga agaacaaatt cagcgattag aagattacaa gcagctgaaa cacttgaatc
     1861 agcatgtgca gcagctgcac aggaggtaag gaagattacg ggatttgaca gagtaatgat
     1921 ctatagattt gctagtgact tcagtggcga agttatagct gaagatagat gtgcggaggt
     1981 tgagagtaaa ctgggtttac attatccagc ttccacagtt ccagcgcaag caaggagatt
     2041 atacactata aacccagtaa ggatcatccc agacattaac tacagaccgg tcccagtaac
     2101 tcctgattta aacccagtaa ccggtagacc tattgacttg tctttcgcaa ttttaaggtc
     2161 tgtgtcccca gtccatttgg agtttatgag aaatataggg atgcatggga cgatgtcaat
     2221 ttctatactg cgtggtgaga gactgtgggg acttattgtt tgccatcata gaactccata
     2281 ttatgtggat ctggacggta gacaggcgtg tgaacttgtg gcccaggtgt tagcttggca
     2341 gatcggagtt atggaagaga aggagggcca cggcaccggc agcaccggca gcggcagcag
     2401 cggcagaagc ggcaccgcca gcagcgagga caacaacatg gccagaatgc tagcatttta
     2461 actcgagggc gcgccacttc taaataagcg aatttcttat gatttatgat ttttattatt
     2521 aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta ggttttaaaa
     2581 cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc tcaggtatag
     2641 tatgaggtcg ctcttattga ccacacctct accggcagat ccgctaggga taacagggta
     2701 atatagatcc atggtcatag ctgtttcctg ataacttcgt ataatgtatg ctatacgaag
     2761 ttattctgta ggcgaaccta accggccaat tttccaagac cgctctgccg ctgagcttgc
     2821 acccgtgccg ctacacaaca cccacacatc cgtaaacccc ccgccatcgg tatcaccaag
     2881 gttgtctatg acgaggcgta caagaggacg tgggggccgc ctctccaatg tcaccaatag
     2941 gaaaaaagaa agaccgagtg cgagtttctg acagaagcct caagaatttt ttttctttct
     3001 tcgactatgc tggaggtaga gatgatcgag gcagtagtca agtatatata aattaattag
     3061 ttccaccacc ttcttttctt gtgttgctcc tttcgatgct atttctggct ttttctaatt
     3121 tttttcatcg tttcttactt tcttcctaat atataaattc ttttgcattt tcattcttcc
     3181 tctccatttg ttcattctcc ttaaattttt tttaggtctc ctttctttca agaaacacag
     3241 tcatcgaact ataattaact aaacatgtca gcacctaaga agatagtagt attacctgga
     3301 gaccacgtag gacaggagat aacagcagag gcaataaagg tattaaaggc aatatcagac
     3361 gtaaggtcaa acgtaaagtt cgacttcgag aaccacttaa taggaggagc agcaatagac
     3421 gcaacaggag tacctttacc tgacgaggca ttagaggcat caaagaaggc agacgcagta
     3481 ttattaggag cagtaggagg acctaagtgg ggaacaggat cagtaaggcc tgagcaggga
     3541 ttattaaaga taaggaagga gttacagtta tacgcaaact taaggccttg caacttcgca
     3601 tcagactcat tattagactt atcacctata aagcctcagt tcgcaaaggg aacagacttc
     3661 gtagtagtaa gggagttagt aggaggaata tacttcggaa agaggaagga ggacgacgga
     3721 gacggagtag catgggactc agagcagtac acagtacctg aggtacagag gataacaagg
     3781 atggcagcat tcatggcatt acagcacgag cctcctttac ctatatggtc attagacaag
     3841 gcaaacgtat tagcatcatc aaggttatgg aggaagacag tagaggagac aataaagaac
     3901 gagttcccta cattaaaggt acagcaccag ttaatagact cagcagcaat gatattagta
     3961 aagaacccta cacacttaaa cggaataata ataacatcaa acatgttcgg agacataata
     4021 tcagacgagg catcagtaat acctggatca ttaggattat taccttcagc atcattagca
     4081 tcattacctg acaagaacac agcattcgga ttatacgagc cttgccacgg atcagcacct
     4141 gacttaccta agaacaaggt aaaccctata gcaacaatat tatcagcagc aatgatgtta
     4201 aagttatcat taaacttacc tgaggaggga aaggcaatag aggacgcagt aaagaaggta
     4261 ttagacgcag gaataaggac aggagactta ggaggatcaa actcaacaac agaggtagga
     4321 gacgcagtag cagaggaggt aaagaagata ttagcatgag aatattgata aatttaaatt
     4381 ttggaatgag ggttgtcctt tcctttacaa tagaggcatt tgagtgagta ggttttatca
     4441 ccttcttgct cttattaaat cgcagttgtc tttccggtat gtgcttgact gaggaacaat
     4501 attttattca tgatacattt ccatgctacg catatcgtca tccacatgga aggtaatttt
     4561 cttctgtggc ctttcatata gttgtgaatg gtagaacaat cttcatataa taacttcgta
     4621 taatgtatgc tatacgaagt tattaatacg actcactata gggcgaattg gagctccaat
     4681 tcggacgtcc cctatagtga gtcgtattat taattaattc gaaggccgcg ttgctggcgt
     4741 ttttccatag gctcggcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     4801 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     4861 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     4921 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     4981 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     5041 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     5101 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     5161 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     5221 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     5281 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     5341 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     5401 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     5461 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     5521 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctgcccgtcg
     5581 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     5641 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     5701 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     5761 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     5821 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     5881 caaggcgagt tacatgatcc cccatgttgt gaaaaaaagc ggttagctcc ttcggtcctc
     5941 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     6001 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     6061 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     6121 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     6181 cggggcgaaa actctcaagg atcttaccgc tgttgaga
//
