LOCUS       sequence-154299-        4296 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene Tagging vector HIS/GFP - Plasmid for superfolder GFP (sfGFP) 
            labeling of endogenous proteins with a HIS selection marker, 
            originating from pSIVh (ID:81091).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTH-g
FEATURES             Location/Qualifiers
     source          1..4296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(199..262)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        289..307
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      1085..1272
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1140..1164)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(1311..1327)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1311..1327)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    1328..1361
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1863..2516
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe his5"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=S. pombe his5
                     /note="yeast auxotrophic marker; corresponds to S. 
                     cerevisiae HIS3"
                     /translation="MGRRAFVERNTNETKISVAIALDKAPLPEESNFIDELITSKHANQ
                     KGEQVIQVDTGIGFLDHMYHALAKHAGWSLRLYSRGDLIIDDHHTAEDTAIALGIAFKQ
                     AMGNFAGVKRFGHAYCPLDEALSRSVVDLSGRPYAVIDLGLKREKVGELSCEMIPHLLY
                     SFSVAAGITLHVTCLYGSNDHHRAESAFKSLAVAMRAATSLTGSSEVPSTKGVL"
     terminator      2522..2719
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    2743..2776
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2777..2795
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2824..2842)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2875..3463)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2955..2974)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3634..4296,1..129,130..198))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4257..4276
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
       61 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
      121 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
      181 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
      241 ttgtctcatg agcggataca tatttgaagt atcgttcgaa ttaattaaaa ttaaccctca
      301 ctaaaggggc atgcgcggcc gctctagaac tatccaaggg tgaagagcta tttactgggg
      361 ttgtacccat tttggtagaa ctggacggag atgtaaacgg acataaattc tctgttagag
      421 gtgagggcga aggcgatgcc accaatggta aattgactct gaagtttata tgcactacgg
      481 gtaaattacc tgttccttgg ccaaccctag taacaacttt gacatatggt gttcaatgtt
      541 tctcaagata cccagaccat atgaaaaggc atgatttctt taaaagtgct atgccagaag
      601 gctacgtgca agagagaact atctccttta aggatgacgg tacgtataaa acacgagcag
      661 aagtgaaatt cgaaggggat acactagtta atcgcatcga attaaagggt atagacttta
      721 aggaagatgg taatattctc ggccataaac ttgagtataa tttcaactcg cataatgtgt
      781 acattacagc tgacaaacaa aagaacggaa ttaaagcgaa ttttaaaatc aggcacaacg
      841 tcgaagatgg gtctgttcaa cttgccgatc attatcagca aaacacccct attggtgatg
      901 gtccagtctt gttacccgat aatcactact taagcacaca gtctagattg tcaaaagatc
      961 cgaatgaaaa gcgtgatcac atggttttat tggaatttgt caccgctgca ggaataactc
     1021 acggaatgga cgagctttat aagatgctag cattttaact cgagggcgcg ccacttctaa
     1081 ataagcgaat ttcttatgat ttatgatttt tattattaaa taagttataa aaaaaataag
     1141 tgtatacaaa ttttaaagtg actcttaggt tttaaaacga aaattcttat tcttgagtaa
     1201 ctctttcctg taggtcaggt tgctttctca ggtatagtat gaggtcgctc ttattgacca
     1261 cacctctacc ggcagatccg ctagggataa cagggtaata tagatccatg gtcatagctg
     1321 tttcctgata acttcgtata atgtatgcta tacgaagtta tgtctgtagg cgaacctaac
     1381 cggccaattt tccaagaccg ctctgccgct gagcttgcac ccgtgccgct acacaacacc
     1441 cacacatccg taaacccccc gccatcggta tcaccaaggt tgtctatgac gaggcgtaca
     1501 agaggacgtg ggggccgcct ctccaatgtc accaatagga aaaaagaaag accgagtgcg
     1561 agtttctgac agaagcctca agaatttttt ttctttcttc gactatgctg gaggtagaga
     1621 tgatcgaggc agtagtcaag tatatataaa ttaattagtt ccaccacctt cttttcttgt
     1681 gttgctcctt tcgatgctat ttctggcttt ttctaatttt tttcatcgtt tcttactttc
     1741 ttcctaatat ataaattctt ttgcattttc attcttcctc tccatttgtt cattctcctt
     1801 aaattttttt taggtctcct ttctttcaag aaacacagtc atcgaactat aattaactaa
     1861 acatgggtag gagggctttt gtagaaagaa atacgaacga aacgaaaatc agcgttgcta
     1921 tcgctttgga caaagctccc ttacctgaag agtcgaattt tattgatgaa cttataactt
     1981 ccaaacatgc aaaccaaaag ggagaacaag taatccaagt agacacggga attggattct
     2041 tggatcacat gtatcatgca ctggctaaac atgcaggctg gagcttacga ctttactcaa
     2101 gaggtgattt aatcattgat gatcatcaca cagcagaaga tactgctatt gcacttggta
     2161 ttgcattcaa gcaggctatg ggtaactttg ccggcgttaa aagatttgga catgcttatt
     2221 gtccacttga cgaagctctt tcaagaagcg tagttgattt gtcgggacgg ccctatgctg
     2281 ttattgattt gggattaaag cgtgaaaagg ttggggaatt gtcctgtgaa atgatccctc
     2341 acttactata ttccttttcg gtagctgctg gaattacttt gcatgttacc tgcttatatg
     2401 gtagtaatga ccatcatcgt gctgaaagcg cttttaaatc tctggctgtt gccatgcgcg
     2461 cggctacaag tcttactgga agttctgaag tcccaagcac gaagggagtg ttgtaatcat
     2521 tactgacaat aaaaagattc ttgttttcaa gaacttgtca tttgtatagt ttttttatat
     2581 tgtagttgtt ctattttaat caaatgttag cgtgatttat attttttttc gcctcgacat
     2641 catctgccca gatgcgaagt taagtgcgca gaaagtaata tcatgcgtca atcgtatgtg
     2701 aatgctggtc gctatactgc tgtcgattcg atactaacgc cgataacttc gtataatgta
     2761 tgctatacga agttattaat acgactcact atagggcgaa ttggagctcc aattcggacg
     2821 tcccctatag tgagtcgtat tattaattaa ttcgaaggcc gcgttgctgg cgtttttcca
     2881 taggctcggc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     2941 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     3001 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     3061 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     3121 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     3181 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     3241 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     3301 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     3361 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     3421 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     3481 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     3541 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     3601 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     3661 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactgcccg tcgtgtagat
     3721 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     3781 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     3841 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     3901 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     3961 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     4021 agttacatga tcccccatgt tgtgaaaaaa agcggttagc tccttcggtc ctccgatcgt
     4081 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     4141 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     4201 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     4261 taccgcgcca catagcagaa ctttaaaagt gctcat
//
