LOCUS       sequence-154304-        4305 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene Tagging vector HIS/YFP - Plasmid for monomeric Citrine 
            (mCitrine) labeling of endogenous proteins with a HIS selection 
            marker, originating from pSIVh (ID:81091).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 4305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTH-y
FEATURES             Location/Qualifiers
     source          1..4305
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(115..178)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        205..223
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             255..965
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLKEF
                     VTAAGITHGMDELYK"
     terminator      1010..1197
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1065..1089)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(1236..1252)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1236..1252)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    1253..1286
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1788..2441
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe his5"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=S. pombe his5
                     /note="yeast auxotrophic marker; corresponds to S. 
                     cerevisiae HIS3"
                     /translation="MGRRAFVERNTNETKISVAIALDKAPLPEESNFIDELITSKHANQ
                     KGEQVIQVDTGIGFLDHMYHALAKHAGWSLRLYSRGDLIIDDHHTAEDTAIALGIAFKQ
                     AMGNFAGVKRFGHAYCPLDEALSRSVVDLSGRPYAVIDLGLKREKVGELSCEMIPHLLY
                     SFSVAAGITLHVTCLYGSNDHHRAESAFKSLAVAMRAATSLTGSSEVPSTKGVL"
     terminator      2447..2644
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    2668..2701
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2702..2720
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2749..2767)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2800..3388)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2880..2899)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3559..4305,1..45,46..114))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4182..4201
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
       61 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
      121 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
      181 tgaagtatcg ttcgaattaa ttaaaattaa ccctcactaa aggggcatgc gcggccgctc
      241 tagaactatc tgcttctaaa ggtgaagaat tattcactgg tgttgtccca attttggttg
      301 aattagatgg tgatgttaat ggtcacaaat tttctgtctc cggtgaaggt gaaggtgatg
      361 ctacttacgg taaattgacc ttaaaattta tttgtactac tggtaaattg ccagttccat
      421 ggccaacctt agtcactact ttaggttatg gtttgatgtg ttttgctaga tacccagatc
      481 atatgaaaca acatgacttt ttcaagtctg ccatgccaga aggttatgtt caagaaagaa
      541 ctattttttt caaagatgac ggtaactaca agaccagagc tgaagtcaag tttgaaggtg
      601 ataccttagt taatagaatc gaattaaaag gtattgattt taaagaagat ggtaacattt
      661 taggtcacaa attggaatac aactataact ctcacaatgt ttacatcatg gctgacaaac
      721 aaaagaatgg tatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggttctgttc
      781 aattagctga ccattatcaa caaaatactc caattggtga tggtccagtc ttgttaccag
      841 acaaccatta cttatcctat caatctaaat tatccaaaga tccaaacgaa aagagagacc
      901 acatggtctt gaaagaattt gttactgctg ctggtattac ccatggtatg gatgaattgt
      961 acaaaggaat gctagcattt taactcgagg gcgcgccact tctaaataag cgaatttctt
     1021 atgatttatg atttttatta ttaaataagt tataaaaaaa ataagtgtat acaaatttta
     1081 aagtgactct taggttttaa aacgaaaatt cttattcttg agtaactctt tcctgtaggt
     1141 caggttgctt tctcaggtat agtatgaggt cgctcttatt gaccacacct ctaccggcag
     1201 atccgctagg gataacaggg taatatagat ccatggtcat agctgtttcc tgataacttc
     1261 gtataatgta tgctatacga agttatgtct gtaggcgaac ctaaccggcc aattttccaa
     1321 gaccgctctg ccgctgagct tgcacccgtg ccgctacaca acacccacac atccgtaaac
     1381 cccccgccat cggtatcacc aaggttgtct atgacgaggc gtacaagagg acgtgggggc
     1441 cgcctctcca atgtcaccaa taggaaaaaa gaaagaccga gtgcgagttt ctgacagaag
     1501 cctcaagaat tttttttctt tcttcgacta tgctggaggt agagatgatc gaggcagtag
     1561 tcaagtatat ataaattaat tagttccacc accttctttt cttgtgttgc tcctttcgat
     1621 gctatttctg gctttttcta atttttttca tcgtttctta ctttcttcct aatatataaa
     1681 ttcttttgca ttttcattct tcctctccat ttgttcattc tccttaaatt ttttttaggt
     1741 ctcctttctt tcaagaaaca cagtcatcga actataatta actaaacatg ggtaggaggg
     1801 cttttgtaga aagaaatacg aacgaaacga aaatcagcgt tgctatcgct ttggacaaag
     1861 ctcccttacc tgaagagtcg aattttattg atgaacttat aacttccaaa catgcaaacc
     1921 aaaagggaga acaagtaatc caagtagaca cgggaattgg attcttggat cacatgtatc
     1981 atgcactggc taaacatgca ggctggagct tacgacttta ctcaagaggt gatttaatca
     2041 ttgatgatca tcacacagca gaagatactg ctattgcact tggtattgca ttcaagcagg
     2101 ctatgggtaa ctttgccggc gttaaaagat ttggacatgc ttattgtcca cttgacgaag
     2161 ctctttcaag aagcgtagtt gatttgtcgg gacggcccta tgctgttatt gatttgggat
     2221 taaagcgtga aaaggttggg gaattgtcct gtgaaatgat ccctcactta ctatattcct
     2281 tttcggtagc tgctggaatt actttgcatg ttacctgctt atatggtagt aatgaccatc
     2341 atcgtgctga aagcgctttt aaatctctgg ctgttgccat gcgcgcggct acaagtctta
     2401 ctggaagttc tgaagtccca agcacgaagg gagtgttgta atcattactg acaataaaaa
     2461 gattcttgtt ttcaagaact tgtcatttgt atagtttttt tatattgtag ttgttctatt
     2521 ttaatcaaat gttagcgtga tttatatttt ttttcgcctc gacatcatct gcccagatgc
     2581 gaagttaagt gcgcagaaag taatatcatg cgtcaatcgt atgtgaatgc tggtcgctat
     2641 actgctgtcg attcgatact aacgccgata acttcgtata atgtatgcta tacgaagtta
     2701 ttaatacgac tcactatagg gcgaattgga gctccaattc ggacgtcccc tatagtgagt
     2761 cgtattatta attaattcga aggccgcgtt gctggcgttt ttccataggc tcggcccccc
     2821 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     2881 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     2941 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     3001 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     3061 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3121 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3181 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3241 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     3301 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     3361 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     3421 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     3481 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     3541 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     3601 tctatttcgt tcatccatag ttgcctgact gcccgtcgtg tagataacta cgatacggga
     3661 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     3721 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     3781 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     3841 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     3901 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     3961 catgttgtga aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     4021 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     4081 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     4141 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     4201 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     4261 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacc
//
