LOCUS       sequence-154333-        4169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene Tagging vector TRP/GFP - Plasmid for superfolder GFP (sfGFP) 
            labeling of endogenous proteins with a TRP selection marker, 
            originating from pSIVt (ID:81092).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTT-g
FEATURES             Location/Qualifiers
     source          1..4169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(199..262)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        289..307
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      1085..1272
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1140..1164)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        1347..1690
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     protein_bind    2616..2649
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2650..2668
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2697..2715)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2748..3336)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2828..2847)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3507..4169,1..129,130..198))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4130..4149
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
       61 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
      121 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
      181 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
      241 ttgtctcatg agcggataca tatttgaagt atcgttcgaa ttaattaaaa ttaaccctca
      301 ctaaaggggc atgcgcggcc gctctagaac tatccaaggg tgaagagcta tttactgggg
      361 ttgtacccat tttggtagaa ctggacggag atgtaaacgg acataaattc tctgttagag
      421 gtgagggcga aggcgatgcc accaatggta aattgactct gaagtttata tgcactacgg
      481 gtaaattacc tgttccttgg ccaaccctag taacaacttt gacatatggt gttcaatgtt
      541 tctcaagata cccagaccat atgaaaaggc atgatttctt taaaagtgct atgccagaag
      601 gctacgtgca agagagaact atctccttta aggatgacgg tacgtataaa acacgagcag
      661 aagtgaaatt cgaaggggat acactagtta atcgcatcga attaaagggt atagacttta
      721 aggaagatgg taatattctc ggccataaac ttgagtataa tttcaactcg cataatgtgt
      781 acattacagc tgacaaacaa aagaacggaa ttaaagcgaa ttttaaaatc aggcacaacg
      841 tcgaagatgg gtctgttcaa cttgccgatc attatcagca aaacacccct attggtgatg
      901 gtccagtctt gttacccgat aatcactact taagcacaca gtctagattg tcaaaagatc
      961 cgaatgaaaa gcgtgatcac atggttttat tggaatttgt caccgctgca ggaataactc
     1021 acggaatgga cgagctttat aagatgctag cattttaact cgagggcgcg ccacttctaa
     1081 ataagcgaat ttcttatgat ttatgatttt tattattaaa taagttataa aaaaaataag
     1141 tgtatacaaa ttttaaagtg actcttaggt tttaaaacga aaattcttat tcttgagtaa
     1201 ctctttcctg taggtcaggt tgctttctca ggtatagtat gaggtcgctc ttattgacca
     1261 cacctctacc ggcagatccg ctagggataa cagggtaata tagatctgtt tagcttgcct
     1321 cgtccccgcc gggtcacccg gcgagcgaca tggaggccca gaataccctc cttgacagtc
     1381 ttgacgtgcg cagctcaggg gcatgatgtg actgtcgccc gtacatttag cccatacatc
     1441 cccatgtata atcatttgca tccatacatt ttgatggccg cacggcgcga agcaaaaatt
     1501 acggctcctc gcctcagacc tgcgagcagg gaaacgctcc cctcacagac gcgttgaatt
     1561 gtccccacgc cgcgcccctg tagagaaata taaaaggtta ggatttgcca ctgaggttct
     1621 tctttcatat acttcctttt aaaatcttgc taggatacag ttctcacatc acatccgaac
     1681 ataaacaacc atgagtgtca taaactttac cggatcgagt ggacctctcg tcaaggtatg
     1741 cgggctccag tccactgaag cagcggagtg tgccctagac tctgatgccg atctcttggg
     1801 cattatctgc gtcccgaata ggaaacgcac cattgatcct gtaatcgcgc gcaagatttc
     1861 ttcgctcgta aaggcctaca agaactcttc aggaacgccg aagtatttag tcggtgtttt
     1921 tcgtaatcag cctaaagagg acgtattggc actcgtaaac gactacggca tagatatagt
     1981 gcagttacat ggggacgaga gctggcagga atatcaagag ttcctgggct tgcctgtcat
     2041 taaacgactg gtttttccga aagattgtaa tatccttttg tcagcagcgt cgcagaaacc
     2101 gcactcattc ataccgctct tcgactcgga agcaggcggt acaggggaac tactggactg
     2161 gaatagtata agtgactggg tcggacggca agagagtccg gagtccttac attttatgct
     2221 ggccgggggt ttaacccccg aaaacgtggg agatgccctc cgattaaatg gggttatcgg
     2281 tgtcgatgta tcaggtgggg tggagacgaa tggcgtgaag gactcaaata aaatcgctaa
     2341 ttttgtgaaa aacgctaaga aatgagaata ttgataaatt taaattttgg aatgagggtt
     2401 gtcctttcct ttacaataga ggcatttgag tgagtaggtt ttatcacctt cttgctctta
     2461 ttaaatcgca gttgtctttc cggtatgtgc ttgactgagg aacaatattt tattcatgat
     2521 acatttccat gctacgcata tcgtcatcca catggaaggt aattttcttc tgtggccttt
     2581 catatagttg tgaatggtag aacaatcttc atataataac ttcgtataat gtatgctata
     2641 cgaagttatt aatacgactc actatagggc gaattggagc tccaattcgg acgtccccta
     2701 tagtgagtcg tattattaat taattcgaag gccgcgttgc tggcgttttt ccataggctc
     2761 ggcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     2821 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     2881 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     2941 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     3001 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     3061 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     3121 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     3181 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     3241 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     3301 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     3361 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     3421 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     3481 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     3541 gcgatctgtc tatttcgttc atccatagtt gcctgactgc ccgtcgtgta gataactacg
     3601 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     3661 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     3721 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     3781 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     3841 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     3901 tgatccccca tgttgtgaaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     3961 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     4021 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     4081 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     4141 ccacatagca gaactttaaa agtgctcat
//
