LOCUS       sequence-154338-        4178 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene Tagging vector TRP/YFP - Plasmid for monomeric Citrine 
            (mCitrine) labeling of endogenous proteins with a TRP selection 
            marker, originating from pSIVt (ID:81092).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTT-y
FEATURES             Location/Qualifiers
     source          1..4178
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(88..151)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        178..196
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             228..938
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLKEF
                     VTAAGITHGMDELYK"
     terminator      983..1170
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1038..1062)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        1245..1588
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     protein_bind    2514..2547
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2548..2566
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2595..2613)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2646..3234)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2726..2745)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3405..4178,1..18,19..87))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4028..4047
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
       61 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
      121 tcagggttat tgtctcatga gcggatacat atttgaagta tcgttcgaat taattaaaat
      181 taaccctcac taaaggggca tgcgcggccg ctctagaact atctgcttct aaaggtgaag
      241 aattattcac tggtgttgtc ccaattttgg ttgaattaga tggtgatgtt aatggtcaca
      301 aattttctgt ctccggtgaa ggtgaaggtg atgctactta cggtaaattg accttaaaat
      361 ttatttgtac tactggtaaa ttgccagttc catggccaac cttagtcact actttaggtt
      421 atggtttgat gtgttttgct agatacccag atcatatgaa acaacatgac tttttcaagt
      481 ctgccatgcc agaaggttat gttcaagaaa gaactatttt tttcaaagat gacggtaact
      541 acaagaccag agctgaagtc aagtttgaag gtgatacctt agttaataga atcgaattaa
      601 aaggtattga ttttaaagaa gatggtaaca ttttaggtca caaattggaa tacaactata
      661 actctcacaa tgtttacatc atggctgaca aacaaaagaa tggtatcaaa gttaacttca
      721 aaattagaca caacattgaa gatggttctg ttcaattagc tgaccattat caacaaaata
      781 ctccaattgg tgatggtcca gtcttgttac cagacaacca ttacttatcc tatcaatcta
      841 aattatccaa agatccaaac gaaaagagag accacatggt cttgaaagaa tttgttactg
      901 ctgctggtat tacccatggt atggatgaat tgtacaaagg aatgctagca ttttaactcg
      961 agggcgcgcc acttctaaat aagcgaattt cttatgattt atgattttta ttattaaata
     1021 agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa
     1081 attcttattc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg tatagtatga
     1141 ggtcgctctt attgaccaca cctctaccgg cagatccgct agggataaca gggtaatata
     1201 gatctgttta gcttgcctcg tccccgccgg gtcacccggc gagcgacatg gaggcccaga
     1261 ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt
     1321 acatttagcc catacatccc catgtataat catttgcatc catacatttt gatggccgca
     1381 cggcgcgaag caaaaattac ggctcctcgc ctcagacctg cgagcaggga aacgctcccc
     1441 tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata aaaggttagg
     1501 atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta ggatacagtt
     1561 ctcacatcac atccgaacat aaacaaccat gagtgtcata aactttaccg gatcgagtgg
     1621 acctctcgtc aaggtatgcg ggctccagtc cactgaagca gcggagtgtg ccctagactc
     1681 tgatgccgat ctcttgggca ttatctgcgt cccgaatagg aaacgcacca ttgatcctgt
     1741 aatcgcgcgc aagatttctt cgctcgtaaa ggcctacaag aactcttcag gaacgccgaa
     1801 gtatttagtc ggtgtttttc gtaatcagcc taaagaggac gtattggcac tcgtaaacga
     1861 ctacggcata gatatagtgc agttacatgg ggacgagagc tggcaggaat atcaagagtt
     1921 cctgggcttg cctgtcatta aacgactggt ttttccgaaa gattgtaata tccttttgtc
     1981 agcagcgtcg cagaaaccgc actcattcat accgctcttc gactcggaag caggcggtac
     2041 aggggaacta ctggactgga atagtataag tgactgggtc ggacggcaag agagtccgga
     2101 gtccttacat tttatgctgg ccgggggttt aacccccgaa aacgtgggag atgccctccg
     2161 attaaatggg gttatcggtg tcgatgtatc aggtggggtg gagacgaatg gcgtgaagga
     2221 ctcaaataaa atcgctaatt ttgtgaaaaa cgctaagaaa tgagaatatt gataaattta
     2281 aattttggaa tgagggttgt cctttccttt acaatagagg catttgagtg agtaggtttt
     2341 atcaccttct tgctcttatt aaatcgcagt tgtctttccg gtatgtgctt gactgaggaa
     2401 caatatttta ttcatgatac atttccatgc tacgcatatc gtcatccaca tggaaggtaa
     2461 ttttcttctg tggcctttca tatagttgtg aatggtagaa caatcttcat ataataactt
     2521 cgtataatgt atgctatacg aagttattaa tacgactcac tatagggcga attggagctc
     2581 caattcggac gtcccctata gtgagtcgta ttattaatta attcgaaggc cgcgttgctg
     2641 gcgtttttcc ataggctcgg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2701 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     2761 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     2821 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     2881 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     2941 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3001 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3061 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     3121 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3181 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3241 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     3301 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     3361 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     3421 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactgccc
     3481 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     3541 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     3601 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     3661 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     3721 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     3781 cgatcaaggc gagttacatg atcccccatg ttgtgaaaaa aagcggttag ctccttcggt
     3841 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     3901 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     3961 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     4021 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     4081 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     4141 actcgtgcac ccaactgatc ttcagcatct tttacttt
//
