LOCUS       sequence-154343-        4166 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene Tagging vector TRP/RFP - Plasmid for monomeric Cherry (mCherry)
            labeling of endogenous proteins with a TRP selection marker, 
            originating from pSIVt (ID:81092).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTT-r
FEATURES             Location/Qualifiers
     source          1..4166
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(109..172)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        199..217
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(379..397)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     647..666
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      992..1179
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1047..1071)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        1254..1597
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     protein_bind    2523..2556
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2557..2575
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2604..2622)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2655..3243)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2735..2754)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3414..4166,1..39,40..108))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4037..4056
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
       61 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
      121 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaagt
      181 atcgttcgaa ttaattaaaa ttaaccctca ctaaaggggc atgcgcggcc gctctagaac
      241 taaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
      301 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
      361 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct
      421 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg
      481 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
      541 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
      601 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
      661 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga
      721 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
      781 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca
      841 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
      901 agggccgcca ctccaccggc ggcatggacg agctgtacaa gggatcttaa atgctagcat
      961 tttaactcga gggcgcgcca cttctaaata agcgaatttc ttatgattta tgatttttat
     1021 tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt
     1081 aaaacgaaaa ttcttattct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt
     1141 atagtatgag gtcgctctta ttgaccacac ctctaccggc agatccgcta gggataacag
     1201 ggtaatatag atctgtttag cttgcctcgt ccccgccggg tcacccggcc agcgacatgg
     1261 aggcccagaa taccctcctt gacagtcttg acgtgcgcag ctcaggggca tgatgtgact
     1321 gtcgcccgta catttagccc atacatcccc atgtataatc atttgcatcc atacattttg
     1381 atggccgcac ggcgcgaagc aaaaattacg gctcctcgct gcagacctgc gagcagggaa
     1441 acgctcccct cacagacgcg ttgaattgtc cccacgccgc gcccctgtag agaaatataa
     1501 aaggttagga tttgccactg aggttcttct ttcatatact tccttttaaa atcttgctag
     1561 gatacagttc tcacatcaca tccgaacata aacaaccatg agtgtcataa actttaccgg
     1621 atcgagtgga cctctcgtca aggtatgcgg gctccagtcc actgaagcag cggagtgtgc
     1681 cctagactct gatgccgatc tcttgggcat tatctgcgtc ccgaatagga aacgcaccat
     1741 tgatcctgta atcgcgcgca agatttcttc gctcgtaaag gcctacaaga actcttcagg
     1801 aacgccgaag tatttagtcg gtgtttttcg taatcagcct aaagaggacg tattggcact
     1861 cgtaaacgac tacggcatag atatagtgca gttacatggg gacgagagct ggcaggaata
     1921 tcaagagttc ctgggcttgc ctgtcattaa acgactggtt tttccgaaag attgtaatat
     1981 ccttttgtca gcagcgtcgc agaaaccgca ctcattcata ccgctcttcg actcggaagc
     2041 aggcggtaca ggggaactac tggactggaa tagtataagt gactgggtcg gacggcaaga
     2101 gagtccggag tccttacatt ttatgctggc cgggggttta acccccgaaa acgtgggaga
     2161 tgccctccga ttaaatgggg ttatcggtgt cgatgtatca ggtggggtgg agacgaatgg
     2221 cgtgaaggac tcaaataaaa tcgctaattt tgtgaaaaac gctaagaaat gagaatattg
     2281 ataaatttaa attttggaat gagggttgtc ctttccttta caatagaggc atttgagtga
     2341 gtaggtttta tcaccttctt gctcttatta aatcgcagtt gtctttccgg tatgtgcttg
     2401 actgaggaac aatattttat tcatgataca tttccatgct acgcatatcg tcatccacat
     2461 ggaaggtaat tttcttctgt ggcctttcat atagttgtga atggtagaac aatcttcata
     2521 taataacttc gtataatgta tgctatacga agttattaat acgactcact atagggcgaa
     2581 ttggagctcc aattcggacg tcccctatag tgagtcgtat tattaattaa ttcgaaggcc
     2641 gcgttgctgg cgtttttcca taggctcggc ccccctgacg agcatcacaa aaatcgacgc
     2701 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     2761 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2821 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     2881 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     2941 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     3001 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     3061 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     3121 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     3181 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     3241 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     3301 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     3361 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     3421 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     3481 tgactgcccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     3541 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     3601 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     3661 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     3721 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     3781 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgaaaaaa agcggttagc
     3841 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     3901 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     3961 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     4021 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     4081 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     4141 atgtaaccca ctcgtgcacc caactg
//
