LOCUS       sequence-154348-        5627 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene Tagging vector TRP/iRFP - Plasmid for tandem infrared RFP 
            (tdiRFP) labeling of endogenous proteins with a TRP selection 
            marker, originating from pSIVt (ID:81092).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5627)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 5627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTT-ir
FEATURES             Location/Qualifiers
     source          1..5627
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(27..90)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        117..135
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     175..191
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     176..192
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     220..236
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     220..236
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      2371..2558
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2426..2450)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        2633..2976
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     protein_bind    3902..3935
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3936..3954
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3983..4001)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(4034..4622)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4114..4133)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4793..5584,5585..5627,1..26))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5416..5435
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
       61 cagggttatt gtctcatgag cggatacata tttgaagtat cgttcgaatt aattaaaatt
      121 aaccctcact aaaggggcat gcaacaaaag ctgggtacgg taccgggccc cccctcgagg
      181 tcgacggtat cgataagctt ctcaagaact ggcggccgct ctagaactag tggatcctct
      241 agaactaatg gcagagggaa gcgttgcccg tcaacccgac ctattaactt gcgatgatga
      301 acctatccat atccccggag caattcaacc tcacggtcta ttgttagccc ttgctgccga
      361 tatgacaatc gttgcaggtt cagataacct accggaattg accgggttag ccattggagc
      421 cttgatcggc cgttcagccg cagatgtgtt cgattcagaa acacataata gactgaccat
      481 cgccttggct gaaccagggg ccgctgttgg cgctcccatt acagttggct tcacgatgag
      541 aaaggatgct ggttttattg gatcatggca tcgtcatgat caacttattt tcttggaatt
      601 ggaacctcct caaagggatg ttgctgaacc tcaggctttc tttcgtcgta ctaatagcgc
      661 tattcgtaga cttcaggctg ctgaaacatt ggagagcgct tgcgcagcag cagctcaaga
      721 agttagaaag attaccggtt ttgacagagt aatgatatat agatttgcgt ccgatttctc
      781 tggtgaagtt attgcggaag atagatgtgc cgaggtagag tcaaagttgg ggttacatta
      841 tcctgcttca acggttccag cacaagcaag gcgtctgtac acaataaacc ccgtaagaat
      901 aattccagac atcaactaca gacctgtccc agtaactcct gatctgaacc ctgtgactgg
      961 tagacctata gatttatctt ttgcaatttt gagatcagtg tcaccggttc acttggagtt
     1021 catgagaaat attggtatgc acggaaccat gtccatttct atactgaggg gcgaaaggtt
     1081 gtgggggctg attgtttgcc atcataggac accatattat gtggacttag acggtaggca
     1141 ggcgtgcgag ctggtcgccc aagttttggc ttggcagatt ggtgtcatgg aagagaagga
     1201 gggccacggc accggcagca ccggcagcgg cagcagcggc agaagcggca ccgccagcag
     1261 cgaggacaac aacatggcca gaatgctaga actaatggcc gaaggtagcg ttgcacgtca
     1321 acccgatcta ctaacatgcg atgatgaacc tatacatatt cctggggcta ttcaacctca
     1381 tggtttgtta ttggcattgg cagctgatat gacgatcgtt gctggaagcg acaatttgcc
     1441 ggagttaact ggactagcca tcggtgctct tataggtaga tccgctgctg acgttttcga
     1501 ttcagaaacg cataacaggc taacgatcgc tcttgctgag cctggtgcag ccgttggagc
     1561 acccatcacc gttggtttta ctatgagaaa ggatgctggt tttattggtt cctggcatag
     1621 acatgaccaa ttaatattct tagaattgga acctccccag agggatgtcg cggaaccaca
     1681 agcatttttt agaagaacaa attcagcgat tagaagatta caagcagctg aaacacttga
     1741 atcagcatgt gcagcagctg cacaggaggt aaggaagatt acgggatttg acagagtaat
     1801 gatctataga tttgctagtg acttcagtgg cgaagttata gctgaagata gatgtgcgga
     1861 ggttgagagt aaactgggtt tacattatcc agcttccaca gttccagcgc aagcaaggag
     1921 attatacact ataaacccag taaggatcat cccagacatt aactacagac cggtcccagt
     1981 aactcctgat ttaaacccag taaccggtag acctattgac ttgtctttcg caattttaag
     2041 gtctgtgtcc ccagtccatt tggagtttat gagaaatata gggatgcatg ggacgatgtc
     2101 aatttctata ctgcgtggtg agagactgtg gggacttatt gtttgccatc atagaactcc
     2161 atattatgtg gatctggacg gtagacaggc gtgtgaactt gtggcccagg tgttagcttg
     2221 gcagatcgga gttatggaag agaaggaggg ccacggcacc ggcagcaccg gcagcggcag
     2281 cagcggcaga agcggcaccg ccagcagcga ggacaacaac atggccagaa tgctagcatt
     2341 ttaactcgag ggcgcgccac ttctaaataa gcgaatttct tatgatttat gatttttatt
     2401 attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta
     2461 aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta
     2521 tagtatgagg tcgctcttat tgaccacacc tctaccggca gatccgctag ggataacagg
     2581 gtaatataga tctgtttagc ttgcctcgtc cccgccgggt cacccggcga gcgacatgga
     2641 ggcccagaat accctccttg acagtcttga cgtgcgcagc tcaggggcat gatgtgactg
     2701 tcgcccgtac atttagccca tacatcccca tgtataatca tttgcatcca tacattttga
     2761 tggccgcacg gcgcgaagca aaaattacgg ctcctcgcct cagacctgcg agcagggaaa
     2821 cgctcccctc acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga gaaatataaa
     2881 aggttaggat ttgccactga ggttcttctt tcatatactt ccttttaaaa tcttgctagg
     2941 atacagttct cacatcacat ccgaacataa acaaccatga gtgtcataaa ctttaccgga
     3001 tcgagtggac ctctcgtcaa ggtatgcggg ctccagtcca ctgaagcagc ggagtgtgcc
     3061 ctagactctg atgccgatct cttgggcatt atctgcgtcc cgaataggaa acgcaccatt
     3121 gatcctgtaa tcgcgcgcaa gatttcttcg ctcgtaaagg cctacaagaa ctcttcagga
     3181 acgccgaagt atttagtcgg tgtttttcgt aatcagccta aagaggacgt attggcactc
     3241 gtaaacgact acggcataga tatagtgcag ttacatgggg acgagagctg gcaggaatat
     3301 caagagttcc tgggcttgcc tgtcattaaa cgactggttt ttccgaaaga ttgtaatatc
     3361 cttttgtcag cagcgtcgca gaaaccgcac tcattcatac cgctcttcga ctcggaagca
     3421 ggcggtacag gggaactact ggactggaat agtataagtg actgggtcgg acggcaagag
     3481 agtccggagt ccttacattt tatgctggcc gggggtttaa cccccgaaaa cgtgggagat
     3541 gccctccgat taaatggggt tatcggtgtc gatgtatcag gtggggtgga gacgaatggc
     3601 gtgaaggact caaataaaat cgctaatttt gtgaaaaacg ctaagaaatg agaatattga
     3661 taaatttaaa ttttggaatg agggttgtcc tttcctttac aatagaggca tttgagtgag
     3721 taggttttat caccttcttg ctcttattaa atcgcagttg tctttccggt atgtgcttga
     3781 ctgaggaaca atattttatt catgatacat ttccatgcta cgcatatcgt catccacatg
     3841 gaaggtaatt ttcttctgtg gcctttcata tagttgtgaa tggtagaaca atcttcatat
     3901 aataacttcg tataatgtat gctatacgaa gttattaata cgactcacta tagggcgaat
     3961 tggagctcca attcggacgt cccctatagt gagtcgtatt attaattaat tcgaaggccg
     4021 cgttgctggc gtttttccat aggctcggcc cccctgacga gcatcacaaa aatcgacgct
     4081 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4141 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4201 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     4261 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4321 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     4381 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     4441 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     4501 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4561 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4621 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4681 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4741 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4801 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     4861 gactgcccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     4921 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     4981 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     5041 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     5101 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     5161 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgaaaaaaa gcggttagct
     5221 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     5281 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     5341 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     5401 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     5461 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     5521 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     5581 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagg
//
