LOCUS       sequence-154364-        8977 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8977)
  AUTHORS   Nemoto S, Fergusson MM, Finkel T
  TITLE     SIRT1 functionally interacts with the metabolic regulator and 
            transcriptional coactivator PGC-1{alpha}.
  JOURNAL   J Biol Chem. 2005 Apr 22. 280(16):16456-60.
  PUBMED    15716268
REFERENCE   2  (bases 1 to 8977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2005 Apr 22. 280(16):16456-60."
COMMENT     Sequence Label: pCruzHA SIRT1
FEATURES             Location/Qualifiers
     source          1..8977
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(262..281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(881..900)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             892..1707
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLVGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(965..984)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     enhancer        2017..2396
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2397..2600
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2551..2571
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2645..2664
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2645..2663
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2686..2695
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2701..2727
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2707..2733
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             2824..5037
                     /codon_start=1
                     /product="mouse NAD+-dependent protein deacetylase 
                     sirtuin-1"
                     /label=SIRT1
                     /note="homolog of budding yeast Sir2"
                     /translation="MADEVALALQAAGSPSAAAAMEAASQPADEPLRKRPRRDGPGLGR
                     SPGEPSAAVAPAAAGCEAASAAAPAALWREAAGAAASAEREAPATAVAGDGDNGSGLRR
                     EPRAADDFDDDEGEEEDEAAAAAAAAAIGYRDNLLLTDGLLTNGFHSCESDDDDRTSHA
                     SSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQIVINILSE
                     PPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCAIPDFRSRDGIYARLAVDFPD
                     LPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNID
                     TLEQVAGIQRILQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIM
                     KPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINR
                     EPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRPQKELVHLS
                     ELPPTPLHISEDSSSPERTVPQDSSVIATLVDQATNNNVNDLEVSESSCVEEKPQEVQT
                     GRNVENINVENPDFKAVGSSTADKNERTSVAETVRKCWPNRLAKEQISKRLEGNQYLFV
                     PPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPLEDESEIEEFYNGLED
                     DTERPECAGGSGFGADGGDQEVVNEAIATRQELTDVNYPSDKS"
     regulatory      2878..2887
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5115..5133)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5116..5133)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5153..5170)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5159..5383
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5429..5857
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5516..5535)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5726..5747
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5866..5886)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5871..6200
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6051..6186
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6113..6132
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6267..7061
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDLATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6321..6340)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6931..6950
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7235..7356
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7272..7291)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7326..7345
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7405..7421)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7405..7421)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7418..7440)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7429..7445
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7453..7459,7460..7477,7478..7483))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7498..7519
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             8541..8732
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(8726..8748)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8834..8973
                     /label=bom
                     /note="basis of mobility region from pBR322"
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat
      901 attcaacggg aaacgtcttg ctctaggccg cgattaaatt ccaacatgga tgctgattta
      961 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgattg
     1021 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat
     1081 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc
     1141 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccggg
     1201 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg
     1261 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc
     1321 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg
     1381 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat
     1441 aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac
     1501 cttatttttg acgaggggaa attagtaggt tgtattgatg ttggacgagt cggaatcgca
     1561 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta
     1621 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt
     1681 catttgatgc tcgatgagtt tttctaagaa ttaattcatg agcggataca tatttgaatg
     1741 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaac ggccgctctc
     1801 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     1861 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
     1921 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
     1981 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
     2041 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     2101 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     2161 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     2221 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     2281 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     2341 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     2401 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcgcgg ggatttccaa
     2461 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     2521 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     2581 aggtctatat aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg
     2641 aaattaatac gactcactat agggagaccc aagcttctcg aggccgccac catgggatcc
     2701 tacccttacg acgttcctga ttacgctagc ctcgaattca gtactgcggc cgcggtaccg
     2761 agctcggatc cactagtcca gtgtggtgga attctgcgga ttcgacagag aggcagttgg
     2821 aagatggcgg acgaggtggc gctcgccctt caggccgccg gctccccttc cgcggcggcc
     2881 gccatggagg ccgcgtcgca gccggcggac gagccgctcc gcaagaggcc ccgccgagac
     2941 gggcctggcc tcgggcgcag cccgggcgag ccgagcgcag cagtggcgcc ggcggccgcg
     3001 gggtgtgagg cggcgagcgc cgcggccccg gcggcgctgt ggcgggaggc ggcaggggcg
     3061 gcggcgagcg cggagcggga ggccccggcg acggccgtgg ccggggacgg agacaatggg
     3121 tccggcctgc ggcgggagcc gagggcggcc gacgacttcg acgacgacga gggcgaggag
     3181 gaggacgagg cggcggcggc agcggcggcg gcagcgatcg gctaccgaga caacctcctg
     3241 ttgaccgatg gactcctcac taatggcttt cattcctgtg aaagtgatga cgatgacaga
     3301 acgtcacacg ccagctctag tgactggact ccgcggccgc ggataggtcc atatactttt
     3361 gttcagcaac atctcatgat tggcaccgat cctcgaacaa ttcttaaaga tttattacca
     3421 gaaacaattc ctccacctga gctggatgat atgacgctgt ggcagattgt tattaatatc
     3481 ctttcagaac caccaaagcg gaaaaaaaga aaagatatca atacaattga agatgctgtg
     3541 aagttactgc aggagtgtaa aaagataata gttctgactg gagctggggt ttctgtctcc
     3601 tgtgcgattc ctgacttcag atcaagagac ggtatctatg ctcgccttgc ggtggacttc
     3661 ccagacctcc cagaccctca agccatgttt gatattgagt attttagaaa agacccaaga
     3721 ccattcttca agtttgcaaa ggaaatatat cccggacagt tccagccgtc tctgtgtcac
     3781 aaattcatag ctttgtcaga taaggaagga aaactacttc gaaattatac tcaaaatata
     3841 gataccttgg agcaggttgc aggaatccaa aggatccttc agtgtcatgg ttcctttgca
     3901 acagcatctt gcctgatttg taagtacaaa gttgattgtg aagctgttcg tggagacatt
     3961 tttaatcagg tagttcctcg gtgccctagg tgcccagctg atgagccact tgccatcatg
     4021 aagccagaga ttgtcttctt tggtgaaaac ttaccagaac agtttcatag agccatgaag
     4081 tatgacaaag atgaagttga cctcctcatt gttattggat cttctctgaa agtgagacca
     4141 gtagcactaa ttccaagttc tataccccat gaagtgcctc aaatattaat aaatagggaa
     4201 cctttgcctc atctacattt tgatgtagag ctccttggag actgcgatgt tataattaat
     4261 gagttgtgtc ataggctagg tggtgaatat gccaaacttt gttgtaaccc tgtaaagctt
     4321 tcagaaatta ctgaaaaacc tccacgccca caaaaggaat tggttcattt atcagagttg
     4381 ccaccaacac ctcttcatat ttcggaagac tcaagttcac ctgaaagaac tgtaccacaa
     4441 gactcttctg tgattgctac acttgtagac caagcaacaa acaacaatgt taatgattta
     4501 gaagtatctg aatcaagttg tgtggaagaa aaaccacaag aagtacagac tggtaggaat
     4561 gttgagaaca ttaatgtgga aaatccagat tttaaggctg ttggttccag tactgcagac
     4621 aaaaatgaaa gaacttcagt tgcagaaaca gtgagaaaat gctggcctaa tagacttgca
     4681 aaggagcaga ttagtaagcg gcttgagggt aatcaatacc tgtttgtacc accaaatcgc
     4741 tacatattcc acggtgctga ggtatactca gactctgaag atgacgtctt gtcctctagt
     4801 tcctgtggca gtaacagtga cagtggcaca tgccagagtc caagtttaga agaacccttg
     4861 gaagatgaaa gtgaaattga agaattctac aatggcttgg aagatgatac ggagaggccc
     4921 gaatgtgctg gaggatctgg atttggagct gatggagggg atcaagaggt tgttaatgaa
     4981 gctatagcta caagacagga attgacagat gtaaactatc catcagacaa atcataacac
     5041 tattgaagct gtccggattc aggaattggt cgaatccagc acagtggcgg ccgctcgagt
     5101 ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc agcctcgact
     5161 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     5221 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     5281 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     5341 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     5401 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     5461 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     5521 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     5581 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     5641 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     5701 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     5761 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     5821 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     5881 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     5941 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     6001 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     6061 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     6121 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     6181 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     6241 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     6301 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     6361 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     6421 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     6481 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     6541 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     6601 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatctg gctacctgcc
     6661 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     6721 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     6781 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     6841 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     6901 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     6961 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     7021 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     7081 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     7141 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     7201 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     7261 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7321 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     7381 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     7441 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     7501 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     7561 gtcgtgccag ctgcattaat gaatcgacca acgcgcgggg agaggcggtt tgcgtattgg
     7621 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     7681 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg atacctcagg
     7741 caattaatgt aagttagctc actcattagg caccgggatc tcgaccgatg cccttgagag
     7801 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
     7861 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt
     7921 tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg
     7981 gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg
     8041 agaagcaggc cattatcgcc ggcatggcgg ccccacgggt gcgcatgatc gtgctcctgt
     8101 cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga
     8161 tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat
     8221 gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca
     8281 ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga acacctacat
     8341 ctgtattaac gaagcgctgg cattgaccct gagtgatttt tctctggtcc cgccgcatcc
     8401 ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa
     8461 cccgtatcgt gagcatcctc tctcgtttca tcggtatcat tacccccatg aacagaaatc
     8521 ccccttacac ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac atggcccgct
     8581 ttatcagaag ccagacatta acgcttctgg agaaactcaa cgagctggac gcggatgaac
     8641 aggcagacat ctgtgaatcg cttcacgacc acgctgatga gctttaccgc agctgcctcg
     8701 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     8761 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     8821 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
     8881 taactatgcg gcatcagagc agattgtact gagagtgcac cattgcggtg tgaaataccg
     8941 cacagatgcg taaggagaaa ataccgcatc aggcgct
//
