LOCUS       sequence-154384-         987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CEN plasmid expressing full-length WHI2 from S.cerevisiae S288C 
            background under its native promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Vijayraghavan S, Kozmin SG, Strope PK, Skelly DA, Lin Z, Kennell J, 
            Magwene PM, Dietrich FS, McCusker JH
  TITLE     Mitochondrial Genome Variation Affects Multiple Respiration and 
            Non-respiration Phenotypes in Saccharomyces cerevisiae.
  JOURNAL   Genetics. 2018 Nov 29. pii: genetics.118.301546. doi: 
            10.1534/genetics.118.301546.
  PUBMED    30498022
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2018 Nov 29. pii: genetics.118.301546. doi: 
            10.1534/genetics.118.301546."
COMMENT     Sequence Label: pWHI2-NAT
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(117..314)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(320..892)
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
ORIGIN
        1 gcgaattgga gctccaccgc ggtggcggcc gcataggcca ctagtggatc tgatatcatc
       61 gatgaattcg agctcgtttt cgacactgga tggcggcgtt agtatcgaat cgacagcagt
      121 atagcgacca gcattcacat acgattgacg catgatatta ctttctgcgc acttaacttc
      181 gcatctgggc agatgatgtc gaggcgaaaa aaaatataaa tcacgctaac atttgattaa
      241 aatagaacaa ctacaatata aaaaaactat acaaatgaca agttcttgaa aacaagaatc
      301 tttttattgt cagtactgat taggggcagg gcatgctcat gtagagcgcc tgctcgccgt
      361 ccgaggcggt gccgtcgtac agggcggtgt ccaggccgca gagggtgaac cccatccgcc
      421 ggtacgcgtg gatcgccggt gcgttgacgt tggtgacctc cagccagagg tgcccggcgc
      481 cccgctcgcg ggcgaactcc gtcgcgagcc ccatcaacgc gcgcccgacc ccgtgccccc
      541 ggtgctccgg ggcgacctcg atgtcctcga cggtcagccg gcggttccag ccggagtacg
      601 agacgaccac gaagcccgcc aggtcgccgt cgtccccgta cgcgacgaac gtccgggagt
      661 ccgggtcgcc gtcctccccg tcgtccgatt cgtcgtccga ttcgtcgtcg gggaacacct
      721 tggtcagggg cgggtccacc ggcacctccc gcagggtgaa gccgtccccg gtggcggtga
      781 cgcggaagac ggtgtcggtg gtgaaggacc catccagtgc ctcgatggcc tcggcgtccc
      841 ccgggacact ggtgcggtac cggtaagccg tgtcgtcaag agtggtaccc atggttgttt
      901 atgttcggat gtgatgtgag aactgtatcc tagcaagatt ttaaaaggaa gtatatgaaa
      961 gaagaacctc agtggcaaat cctaacc
//
