LOCUS       sequence-154394-         647 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PCR template for amplifying the attL1-em7-kanr-attL2-pA cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Zhao L, Ng ET, Koopman P
  TITLE     A piggyBac transposon- and gateway-enhanced system for efficient BAC
            transgenesis.
  JOURNAL   Dev Dyn. 2014 Jun 12. doi: 10.1002/dvdy.24153.
  PUBMED    24924516
REFERENCE   2  (bases 1 to 647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2014 Jun 12. doi: 10.1002/dvdy.24153."
COMMENT     Sequence Label: pBSWt1-Dest
FEATURES             Location/Qualifiers
     source          1..647
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(379..396)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(543..562)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     597..616
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gtgttaatcc ctcattccaa agctccctac tgcttctggt gggcctggaa tgagccaggg
       61 cccaccgaga gctggaagtt cttcgggtcg gctcttcggg cttgtcagag gcagcacggg
      121 ggcggggggt gggggatgtc cctaaaccac agcaccactc ggagtcctca tcttggtcta
      181 ccctagacgt tttaagagtc aacgcctggt gaggccttat gcatcagact ttcccccggt
      241 gtagagtcac acgttacagc caacctcctc ttcctcctcc cgtggagttc cgaaatctct
      301 aggacacccc ggggccccag atgtcctccc gcgtcctacg aactatcgga ctctggggtt
      361 gcgctcagta atgtgtaagt ggtggtggtg gtggtgcgtt gtcgcgttgt cactcgcatt
      421 tgacagagcc cactcctccc tgtccgctac tttcagactc ccttggaagg ctgctgagta
      481 agaactggca tcaggaagga acagacaagg ggagaagcgg cgttaattaa ttaagataca
      541 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
      601 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaa
//
