LOCUS       sequence-154396-         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  recipient plasmid [multiplexing]; pICU2:Cas9, FAST, BASTA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE151
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(50..152)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
       61 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
      121 ggtgaaagtt ggaacctctt acgtgccgat caacgtctcg gtctctgcaa gaattcaagc
      181 ttggagttaa tagatgttta gtttttatta tgacaaaaaa gttgcagaga atgtaacctt
      241 tacttggctt ttgcttttgt taaagtggtt ttaggtggtc ggcaacacat catctctcat
      301 cattgttttt ttttcttttc tctttcaatt gggctttatc atagagaaca atgattttgg
      361 atatgaactt attagtagtt tcagcaatag tttagacttt cgaatcggta aatatcagta
      421 acttagaact caggctgaaa ctttgattct tttactgtat ttagctagat tctacacttg
      481 gtggactcca tcagcaatta cttcacagac cgaggtagct cctacaactc tctgatgctt
      541 gatctcgttc aaatagatgt ctcataattg cttctttttt gtttttgttt ttgggcttta
      601 ttaaagagga gtaaggccca ttaagagaac ccgtttgttt gttggattcg gatttggcaa
      661 atggatagcg ccaaaatcgt gtcgattttc ccgccatctc catttcttaa tctgggttct
      721 taaacctaaa tcagttactc agcttcacca aaccaccaaa ttgaccggat ttgtaaaaat
      781 ggacaagaag tactccattg ggctcgatat cggcacaaac agcgtcggct gggccgtcat
      841 tacggacgag tacaaggtgc cgagcaaaaa attcaaagtt ctgggcaata ccgatcgcca
      901 cagcataaag aagaacctca ttggcgccct cctgttcgac tccggggaga cggccgaagc
      961 cacgcggctc aaaagaacag c
//
