LOCUS       sequence-154399-         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  recipient plasmid [multiplexing]; pICU2:Cas9, FAST, Hygro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE169
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(52..154)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
       61 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
      121 atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cggtctctgc aagaattcaa
      181 gcttggagtt aatagatgtt tagtttttat tatgacaaaa aagttgcaga gaatgtaacc
      241 tttacttggc ttttgctttt gttaaagtgg ttttaggtgg tcggcaacac atcatctctc
      301 atcattgttt ttttttcttt tctctttcaa ttgggcttta tcatagagaa caatgatttt
      361 ggatatgaac ttattagtag tttcagcaat agtttagact ttcgaatcgg taaatatcag
      421 taacttagaa ctcaggctga aactttgatt cttttactgt atttagctag attctacact
      481 tggtggactc catcagcaat tacttcacag accgaggtag ctcctacaac tctctgatgc
      541 ttgatctcgt tcaaatagat gtctcataat tgcttctttt ttgtttttgt ttttgggctt
      601 tattaaagag gagtaaggcc cattaagaga acccgtttgt ttgttggatt cggatttggc
      661 aaatggatag cgccaaaatc gtgtcgattt tcccgccatc tccatttctt aatctgggtt
      721 cttaaaccta aatcagttac tcagcttcac caaaccacca aattgaccgg atttgtaaaa
      781 atggacaaga agtactccat tgggctcgat atcggcacaa acagcgtcgg ctgggccgtc
      841 attacggacg agtacaaggt gccgagcaaa aaa
//
