LOCUS       sequence-154401-        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  recipient plasmid [multiplexing]; p35S:Cas9, FAST, nptII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE160
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    99..154
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             156..950
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(210..229)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     820..839
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa
       61 ttagagtctc atattcactc tcatactgta tttttacaac aattaccaac aacaacaaac
      121 aacaaacaac attacaatta ctatttacaa ttacaatgat tgaacaagat ggattgcacg
      181 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
      241 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
      301 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
      361 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
      421 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
      481 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
      541 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
      601 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
      661 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga tgatctcgtc gtgacccatg
      721 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
      781 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
      841 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
      901 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcttgtcaag
      961 cagatcgttc aaacatttgg caataaagtt tcttaagatt g
//
