LOCUS       sequence-154403-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Multisite gateway entry clone for adding N-terminal fusions of 
            TagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan 
            A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ
  TITLE     A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous 
            System and Neurodegenerative Disease.
  JOURNAL   Zebrafish. 2016 Sep 15.
  PUBMED    27631880
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Zebrafish. 
            2016 Sep 15."
COMMENT     Sequence Label: pME-TagBFP-NS
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..112
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(114..815)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLN"
     regulatory      812..821
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcagctggat ggcaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa
       61 attgataagc aatgctttct tataatgcca actttgtaca agaaagctgg gtcattaagc
      121 ttgtgcccca gtttgctagg gaggtcgcag tatctggcca ctgccacctc gtgctgctcg
      181 acgtaggtct cgttgttggc ctccttgatt ctttccagtc tgtagtccac atagtagacg
      241 ccaggcatct tgaggttctt agcgggtttc ttggatctat atgtggtctt gatgtttgcg
      301 atcagatggc tcccgcccac gagcttcagg gccatgtcgt ttctgccttc caggccgccg
      361 tcagcggggt acagcgtctc ggtgaaggcc tcccagccga gtgttttctt ctgcatcaca
      421 gggccgttgg atgtgaagtt cacccctctg atcttgacgt tgtagatgag gcagccgtcc
      481 tggaggctgg tgtcctgggt agcggtcagc acgcccccgt cttcgtatgt ggtgactctc
      541 tcccatgtga agccctcagg gaaggactgc ttgaagaagt cggggatgcc ctgggtgtgg
      601 ttgatgaagg tcttgctgcc gtagaggaag ctagtagcca ggatgtcgaa ggcgaagggg
      661 agagggccgc cctcgaccac cttgattctc atggtctggg tgccctcgta gggcttgcct
      721 tcgccctcgg atgtgcactt gaagtgatgg ttgtccacgg tgccctccat gtacagcttc
      781 atgtgcatgt tctccttaat cagctcgctc accatggtgg cag
//
