LOCUS       sequence-154404-        1003 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  recipient plasmid [multiplexing]; p35S:Cas9, FAST, nptII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE160
FEATURES             Location/Qualifiers
     source          1..1003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(52..154)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     promoter        593..938
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     881..901
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
       61 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
      121 atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cggtctctgc aagaattcaa
      181 gcttggaggt caacatggtg gagcacgaca ctctggtcta ctccaaaaat gtcaaagata
      241 cagtctcaga agatcaaagg gctattgaga cttttcaaca aaggataatt tcgggaaacc
      301 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
      361 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatctctctg
      421 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagagg
      481 ttccaaccac gtctacaaag caagtggatt gatgtgataa catggtggag cacgacactc
      541 tggtctactc caaaaatgtc aaagatacag tctcagaaga tcaaagggct attgagactt
      601 ttcaacaaag gataatttcg ggaaacctcc tcggattcca ttgcccagct atctgtcact
      661 tcatcgaaag gacagtagaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag
      721 gaaaggctat cattcaagat ctctctgccg acagtggtcc caaagatgga cccccaccca
      781 cgaggagcat cgtggaaaaa gaagaggttc caaccacgtc tacaaagcaa gtggattgat
      841 gtgacatctc cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt
      901 cctctatata aggaagttca tttcatttgg agaggacacg ctcgagtata agagctcatt
      961 tttacaacaa ttaccaacaa caacaaacaa caaacaacat tac
//
