LOCUS       sequence-154410-         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  recipient plasmid [single nuclease]; pPcUbi:Cas9, nptII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE64
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(50..152)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
       61 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
      121 ggtgaaagtt ggaacctctt acgtgccgat caacgtctca gaagacttca atcactactt
      181 cgactctagc tgtatataaa ctcagcttcg ttttcttatc taagcgatgt gggacttttg
      241 aagattgttt tcaacttagc aagaattcaa gcttggagat tacggatatg aatataggca
      301 tatccgtatc cgaattatcc gtttgacagc tagcaacgat tgtacaattg cttctttaaa
      361 aaaggaagaa agaaagaaag aaaagaatca acatcagcgt taacaaacgg ccccgttacg
      421 gcccaaacgg tcatatagag taacggcgtt aagcgttgaa agactcctat cgaaatacgt
      481 aaccgcaaac gtgtcatagt cagatcccct cttccttcac cgcctcaaac acaaaaataa
      541 tcttctacag cctatatata caaccccccc ttctatctct cctttctcac aattcatcat
      601 ctttctttct ctacccccaa ttttaagaaa tcctctcttc tcctcttcat tttcaaggta
      661 aatctctctc tctctctctc tctctgttat tccttgtttt aattaggtat gtattattgc
      721 tagtttgtta atctgcttat cttatgtatg ccttatgtga atatctttat cttgttcatc
      781 tcatccgttt agaagctata aatttgttga tttgactgtg tatctacacg tggttatgtt
      841 tatatctaat cagatatgaa tttcttcata ttgttgcgtt tgtgtgtacc aatccgaaat
      901 cgttgatttt tttcatttaa tcgtgtagct aattgtacgt atacatatgg atctacgtat
      961 caattgttca tctgtttgtg tttgta
//
