LOCUS       sequence-154411-         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  recipient plasmid [single nuclease]; pPcUbi:Cas9, nptII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE64
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(63..246)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(99..278)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atccagatcc
       61 ggtgcagatt atttggattg agagtgaata tgagactcta attggatacc gaggggaatt
      121 tatggaacgt cagtggagca tttttgacaa gaaatatttg ctagctgata gtgaccttag
      181 gcgacttttg aacgcgcaat aatggtttct gacgtatgtg cttagctcat taaactccag
      241 aaacccgcgg ctgagtggct ccttcaacgt tgcggttctg tcagttccaa acgtaaaacg
      301 gcttgtcccg cgtcatcggc gggggtcata acgtgactcc cttaattctc cgctcatgat
      361 tagtagcggt cctgctgagc ctcgacatgt tgtcgcaaaa ttcgccctgg acccgcccaa
      421 cgatttgtcg tcactgtcaa ggtttgacct gcacttcatt tggggcccac atacaccaaa
      481 aaaatgctgc ataattctcg gggcagcaag tcggttaccc ggccgccgtg ctggaccggg
      541 ttgaatggtg cccgtaactt tcggtagagc ggacggccaa tactcaactt caaggaatct
      601 cacccatgcg cgccggcggg gaaccggagt tcccttcagt gagcgttatt agttcgccgc
      661 tcggtgtgtc gtagatacta gcccctgggg cacttttgaa atttgaataa gatttatgta
      721 atcagtcttt taggtttgac cggttctgcc gcttttttta aaattggatt tgtaataata
      781 aaacgcaatt gtttgttatt gtggcgctct atcatagatg tcgctataaa cctattcagc
      841 acaatatatt gttttcattt taatattgta catataagta gtagggtaca atcagtaaat
      901 tgaacggaga atattattca taaaaatacg atagtaacgg gtgatatatt cattagaatg
      961 aaccgaaacc ggcggtaagg atctgagcta c
//
