LOCUS       sequence-154421-        4757 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene Tagging vector LEU/RFP - Plasmid for monomeric Cherry (mCherry)
            labeling of endogenous proteins with a LEU selection marker, 
            originating from pSIVl (ID:81090).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTL-r
FEATURES             Location/Qualifiers
     source          1..4757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(71..134)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        161..179
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(341..359)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     609..628
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      954..1141
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1009..1033)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(1180..1196)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1180..1196)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    1197..1230
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    3076..3109
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3110..3128
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3157..3175)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3208..3796)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3288..3307)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3967..4757,1,2..70))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4590..4609
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
       61 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
      121 tgagcggata catatttgaa gtatcgttcg aattaattaa aattaaccct cactaaaggg
      181 gcatgcgcgg ccgctctaga actaaagggc gaggaggata acatggccat catcaaggag
      241 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
      301 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
      361 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
      421 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
      481 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
      541 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
      601 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
      661 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
      721 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
      781 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
      841 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
      901 aagggatctt aaatgctagc attttaactc gagggcgcgc cacttctaaa taagcgaatt
      961 tcttatgatt tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat
     1021 tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac tctttcctgt
     1081 aggtcaggtt gctttctcag gtatagtatg aggtcgctct tattgaccac acctctaccg
     1141 gcagatccgc tagggataac agggtaatat agatccatgg tcatagctgt ttcctgataa
     1201 cttcgtataa tgtatgctat acgaagttat tctgtaggcg aacctaaccg gccaattttc
     1261 caagaccgct ctgccgctga gcttgcaccc gtgccgctac acaacaccca cacatccgta
     1321 aaccccccgc catcggtatc accaaggttg tctatgacga ggcgtacaag aggacgtggg
     1381 ggccgcctct ccaatgtcac caataggaaa aaagaaagac cgagtgcgag tttctgacag
     1441 aagcctcaag aatttttttt ctttcttcga ctatgctgga ggtagagatg atcgaggcag
     1501 tagtcaagta tatataaatt aattagttcc accaccttct tttcttgtgt tgctcctttc
     1561 gatgctattt ctggcttttt ctaatttttt tcatcgtttc ttactttctt cctaatatat
     1621 aaattctttt gcattttcat tcttcctctc catttgttca ttctccttaa atttttttta
     1681 ggtctccttt ctttcaagaa acacagtcat cgaactataa ttaactaaac atgtcagcac
     1741 ctaagaagat agtagtatta cctggagacc acgtaggaca ggagataaca gcagaggcaa
     1801 taaaggtatt aaaggcaata tcagacgtaa ggtcaaacgt aaagttcgac ttcgagaacc
     1861 acttaatagg aggagcagca atagacgcaa caggagtacc tttacctgac gaggcattag
     1921 aggcatcaaa gaaggcagac gcagtattat taggagcagt aggaggacct aagtggggaa
     1981 caggatcagt aaggcctgag cagggattat taaagataag gaaggagtta cagttatacg
     2041 caaacttaag gccttgcaac ttcgcatcag actcattatt agacttatca cctataaagc
     2101 ctcagttcgc aaagggaaca gacttcgtag tagtaaggga gttagtagga ggaatatact
     2161 tcggaaagag gaaggaggac gacggagacg gagtagcatg ggactcagag cagtacacag
     2221 tacctgaggt acagaggata acaaggatgg cagcattcat ggcattacag cacgagcctc
     2281 ctttacctat atggtcatta gacaaggcaa acgtattagc atcatcaagg ttatggagga
     2341 agacagtaga ggagacaata aagaacgagt tccctacatt aaaggtacag caccagttaa
     2401 tagactcagc agcaatgata ttagtaaaga accctacaca cttaaacgga ataataataa
     2461 catcaaacat gttcggagac ataatatcag acgaggcatc agtaatacct ggatcattag
     2521 gattattacc ttcagcatca ttagcatcat tacctgacaa gaacacagca ttcggattat
     2581 acgagccttg ccacggatca gcacctgact tacctaagaa caaggtaaac cctatagcaa
     2641 caatattatc agcagcaatg atgttaaagt tatcattaaa cttacctgag gagggaaagg
     2701 caatagagga cgcagtaaag aaggtattag acgcaggaat aaggacagga gacttaggag
     2761 gatcaaactc aacaacagag gtaggagacg cagtagcaga ggaggtaaag aagatattag
     2821 catgagaata ttgataaatt taaattttgg aatgagggtt gtcctttcct ttacaataga
     2881 ggcatttgag tgagtaggtt ttatcacctt cttgctctta ttaaatcgca gttgtctttc
     2941 cggtatgtgc ttgactgagg aacaatattt tattcatgat acatttccat gctacgcata
     3001 tcgtcatcca catggaaggt aattttcttc tgtggccttt catatagttg tgaatggtag
     3061 aacaatcttc atataataac ttcgtataat gtatgctata cgaagttatt aatacgactc
     3121 actatagggc gaattggagc tccaattcgg acgtccccta tagtgagtcg tattattaat
     3181 taattcgaag gccgcgttgc tggcgttttt ccataggctc ggcccccctg acgagcatca
     3241 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     3301 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     3361 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     3421 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     3481 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     3541 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     3601 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     3661 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     3721 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     3781 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     3841 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     3901 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     3961 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     4021 atccatagtt gcctgactgc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     4081 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     4141 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     4201 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     4261 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     4321 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgaaa
     4381 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     4441 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     4501 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     4561 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     4621 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     4681 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     4741 caccagcgtt tctgggt
//
