LOCUS       sequence-154427-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  recipient plasmid [multiplexing]; pICU2:Cas9, FAST, nptII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE165
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(38..140)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
       61 atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg
      121 aacctcttac gtgccgatca acgtctcggt ctctgcaaga attcaagctt ggagttaata
      181 gatgtttagt ttttattatg acaaaaaagt tgcagagaat gtaaccttta cttggctttt
      241 gcttttgtta aagtggtttt aggtggtcgg caacacatca tctctcatca ttgttttttt
      301 ttcttttctc tttcaattgg gctttatcat agagaacaat gattttggat atgaacttat
      361 tagtagtttc agcaatagtt tagactttcg aatcggtaaa tatcagtaac ttagaactca
      421 ggctgaaact ttgattcttt tactgtattt agctagattc tacacttggt ggactccatc
      481 agcaattact tcacagaccg aggtagctcc tacaactctc tgatgcttga tctcgttcaa
      541 atagatgtct cataattgct tcttttttgt ttttgttttt gggctttatt aaagaggagt
      601 aaggcccatt aagagaaccc gtttgtttgt tggattcgga tttggcaaat ggatagcgcc
      661 aaaatcgtgt cgattttccc gccatctcca tttcttaatc tgggttctta aacctaaatc
      721 agttactcag cttcaccaaa ccaccaaatt gaccggattt gtaaaaatgg acaagaagta
      781 ctccattggg ctcgatatcg gcacaaacag cgtcggctgg gccgtcatta cggacgagta
      841 caaggtgccg agcaaa
//
