LOCUS       sequence-154430-        8646 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast expression vector p415GPD modified such that it is Gateway 
            converted and introduces a 3xHA tag at the N-terminus of the insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8646)
  AUTHORS   McCann KL, Charette JM, Vincent NG, Baserga SJ
  TITLE     A protein interaction map of the LSU processome.
  JOURNAL   Genes Dev. 2015 Apr 15;29(8):862-75. doi: 10.1101/gad.256370.114. 
            Epub 2015 Apr 15.
  PUBMED    25877921
REFERENCE   2  (bases 1 to 8646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.256370.114"; journalName: "Genes Dev"; date: 
            "2015-04-15- 15"; volume: "29"; issue: "8"; pages: "862-75"
COMMENT     Sequence Label: p415GPD-3xHA-GW
FEATURES             Location/Qualifiers
     source          1..8646
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    70..573
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        610..714
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(715..783,784..1575)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(933..952)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1746..2334
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2235..2254
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2488..2505
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(2658..2663,2664..2681,2682..2688)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2696..2712
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2701..2723
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2720..2736
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2720..2736
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2755..2775
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2757..2775
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2803..3444
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     3338..3361
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             3463..3552
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     protein_bind    3556..3680
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3705..3710,3711..3728,3729..3735)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3789..4448
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3855..3874)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4790..5095
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4974..4993
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5136..5260)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      5263..5510
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(5279..5297)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(5528..5547)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5529..5547)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5557..5574)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5557..5573)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5566..5588)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5718..6173
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5805..5824)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6015..6036
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6473..6877
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             6890..7984
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(8477..8496)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8596..8618
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata
      121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt
      181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa
      241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata
      301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct
      361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata
      421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact
      481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa
      541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg
      601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1861 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     2521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     2581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttacctcact
     2641 cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg tggaattgtg
     2701 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     2761 aaccctcact aaagggaaca aaagctggag ctctcgagtt tatcattatc aatactgcca
     2821 tttcaaagaa tacgtaaata attaatagta gtgattttcc taactttatt tagtcaaaaa
     2881 attagccttt taattctgct gtaacccgta catgccaaaa tagggggcgg gttacacaga
     2941 atatataaca ctgatggtgc ttgggtgaac aggtttattc ctggcatcca ctaaatataa
     3001 tggagcccgc tttttaagct ggcatccaga aaaaaaaaga atcccagcac caaaatattg
     3061 ttttcttcac caaccatcag ttcataggtc cattctctta gcgcaactac agagaacagg
     3121 gcacaaacag gcaaaaaacg ggcacaacct caatggagtg atgcaacctg cctggagtaa
     3181 atgatgacac aaggcaattg acccacgcat gtatctatct cattttctta caccttctat
     3241 taccttctgc tctctctgat ttggaaaaag ctgaaaaaaa aggtttaaac cagttccctg
     3301 aaattattcc cctacttgac taataagtat ataaagacgg taggtattga ttgtaattct
     3361 gtaaatctat ttcttaaact tcttaaattc tacttttata gttagtcttt tttttagttt
     3421 taaaacacca gaacttagtt tcgacggatt ctagatctca tgtacccata cgatgttcct
     3481 gactatgcgg gctatcccta tgacgtcccg gactatgcag gatcctatcc atatgacgtt
     3541 ccagattacg ctggcacaag tttgtacaaa aaagctgaac gagaaacgta aaatgatata
     3601 aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca
     3661 caacatatcc agtcactatg gcggccgcat taggcacccc aggctttaca ctttatgctt
     3721 ccggctcgta taatgtgtgg attttgagtt aggatccgtc gagattttca ggagctaagg
     3781 aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc
     3841 gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc
     3901 agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg
     3961 cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga
     4021 aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc
     4081 aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac
     4141 acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt
     4201 ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt
     4261 taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata
     4321 cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg
     4381 gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg
     4441 gggcgtaaag atctggatcc ggcttactaa aagccagata acagtatgcg tatttgcgcg
     4501 ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg tcaaaaagag
     4561 gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa
     4621 ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt
     4681 cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg
     4741 tttattgaaa tgaacggctc ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa
     4801 ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat
     4861 tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga
     4921 taaagtctcc cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat
     4981 gaccaccgat atggccagtg tgccggtttc cgttatcggg gaagaagtgg ctgatctcag
     5041 ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa tataaatgtc
     5101 aggctccctt atacacagcc agtctgcagg tcgaccatag tgactggata tgttgtgttt
     5161 tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat
     5221 cattttacgt ttctcgttca gctttcttgt acaaagtggt agtcatgtaa ttagttatgt
     5281 cacgcttaca ttcacgccct ccccccacat ccgctctaac cgaaaaggaa ggagttagac
     5341 aacctgaagt ctaggtccct atttattttt ttatagttat gttagtatta agaacgttat
     5401 ttatatttca aatttttctt ttttttctgt acagacgcgt gtacgcatgt aacattatac
     5461 tgaaaacctt gcttgagaag gttttgggac gctcgaaggc tttaatttgc ggccggtacc
     5521 caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg
     5581 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     5641 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     5701 gaatggcgaa tggcgcgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     5761 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     5821 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     5881 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     5941 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     6001 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     6061 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     6121 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa tttcctgatg
     6181 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatcgac ggtcgaggag
     6241 aacttctagt atatccacat acctaatatt attgccttat taaaaatgga atcccaacaa
     6301 ttacatcaaa atccacattc tcttcaaaat caattgtcct gtacttcctt gttcatgtgt
     6361 gttcaaaaac gttatattta taggataatt atactctatt tctcaacaag taattggttg
     6421 tttggccgag cggtctaagg cgcctgattc aagaaatatc ttgaccgcag ttaactgtgg
     6481 gaatactcag gtatcgtaag atgcaagagt tcgaatctct tagcaaccat tatttttttc
     6541 ctcaacataa cgagaacaca caggggcgct atcgcacaga atcaaattcg atgactggaa
     6601 attttttgtt aatttcagag gtcgcctgac gcatatacct ttttcaactg aaaaattggg
     6661 agaaaaagga aaggtgagag gccggaaccg gcttttcata tagaatagag aagcgttcat
     6721 gactaaatgc ttgcatcaca atacttgaag ttgacaatat tatttaagga cctattgttt
     6781 tttccaatag gtggttagca atcgtcttac tttctaactt ttcttacctt ttacatttca
     6841 gcaatatata tatatatttc aaggatatac cattctaatg tctgccccta tgtctgcccc
     6901 taagaagatc gtcgttttgc caggtgacca cgttggtcaa gaaatcacag ccgaagccat
     6961 taaggttctt aaagctattt ctgatgttcg ttccaatgtc aagttcgatt tcgaaaatca
     7021 tttaattggt ggtgctgcta tcgatgctac aggtgtccca cttccagatg aggcgctgga
     7081 agcctccaag aaggttgatg ccgttttgtt aggtgctgtg ggtggtccta aatggggtac
     7141 cggtagtgtt agacctgaac aaggtttact aaaaatccgt aaagaacttc aattgtacgc
     7201 caacttaaga ccatgtaact ttgcatccga ctctctttta gacttatctc caatcaagcc
     7261 acaatttgct aaaggtactg acttcgttgt tgtcagagaa ttagtgggag gtatttactt
     7321 tggtaagaga aaggaagacg atggtgatgg tgtcgcttgg gatagtgaac aatacaccgt
     7381 tccagaagtg caaagaatca caagaatggc cgctttcatg gccctacaac atgagccacc
     7441 attgcctatt tggtccttgg ataaagctaa tgttttggcc tcttcaagat tatggagaaa
     7501 aactgtggag gaaaccatca agaacgaatt ccctacattg aaggttcaac atcaattgat
     7561 tgattctgcc gccatgatcc tagttaagaa cccaacccac ctaaatggta ttataatcac
     7621 cagcaacatg tttggtgata tcatctccga tgaagcctcc gttatcccag gttccttggg
     7681 tttgttgcca tctgcgtcct tggcctcttt gccagacaag aacaccgcat ttggtttgta
     7741 cgaaccatgc cacggttctg ctccagattt gccaaagaat aaggttgacc ctatcgccac
     7801 tatcttgtct gctgcaatga tgttgaaatt gtcattgaac ttgcctgaag aaggtaaggc
     7861 cattgaagat gcagttaaaa aggttttgga tgcaggtatc agaactggtg atttaggtgg
     7921 ttccaacagt accaccgaag tcggtgatgc tgtcgccgaa gaagttaaga aaatccttgc
     7981 ttaaaaagat tctctttttt tatgatattt gtacataaac tttataaatg aaattcataa
     8041 tagaaacgac acgaaattac aaaatggaat atgttcatag ggtagacgaa actatatacg
     8101 caatctacat acatttatca agaaggagaa aaaggaggat agtaaaggaa tacaggtaag
     8161 caaattgata ctaatggctc aacgtgataa ggaaaaagaa ttgcacttta acattaatat
     8221 tgacaaggag gagggcacca cacaaaaagt taggtgtaac agaaaatcat gaaactacga
     8281 ttcctaattt gatattggag gattttctct aaaaaaaaaa aaatacaaca aataaaaaac
     8341 actcaatgac ctgaccattt gatggagttt aagtcaatac cttcttgaac catttcccat
     8401 aatggtgaaa gttccctcaa gaattttact ctgtcagaaa cggccttacg acgtagtcga
     8461 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     8521 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     8581 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     8641 gcgcga
//
