LOCUS       sequence-154448-       16092 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16092)
  AUTHORS   Sen GL, Reuter JA, Webster DE, Zhu L, Khavari PA
  TITLE     DNMT1 maintains progenitor function in self-renewing somatic tissue.
  JOURNAL   Nature. 2010 Jan 28. 463(7280):563-7.
  PUBMED    20081831
REFERENCE   2  (bases 1 to 16092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Jan 28. 463(7280):563-7."
COMMENT     Sequence Label: LZRS-DNMT1
FEATURES             Location/Qualifiers
     source          1..16092
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             349..818
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    883..1240
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1305..1721
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1651..1673
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1689..1705
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1731..2105
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     2043..2059
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     primer_bind     complement(8197..8214)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8368..8956)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8448..8467)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    9079..9213
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9103..9122)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9157..9176
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(9443..10042)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9526..9546)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     10023..10042
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(10125..10624)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10173..10192)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     10566..10589
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     rep_origin      12656..14445
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(join(14939..15730,15731..15799))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15562..15581
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15800..15904)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcaatgtgca tgaatgctgc aatattcctg ttaccaaagc tagtataaat aaaaatagat
       61 aaacgtggaa attacttaga gtttctgtca ttaacgtttc cttcctcagt tgacaacata
      121 aatgcgctgc tgagaagcca gtttgcatct gtcaggatca atttcccatt atgccagtca
      181 tattaattac tagtcaatta gttgattttt atttttgaca tatacatgtg aaagacccca
      241 cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaaatacat
      301 aactgagaat agaaaagttc agatcaaggt caggaacaga tggaacagct gaatatgggc
      361 caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggaa
      421 cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc
      481 caagaacaga tggtccccag atgcggtcca gccctcagca gtttctagag aaccatcaga
      541 tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc
      601 agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac
      661 aacccctcac tcggcgcgcc agtcctccga ttgactgagt cgcccgggta cccgtgtatc
      721 caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc
      781 ctctgagtga ttgactaccc gtcagcgggg gtctttcatt tgggggctcg tccgggatcg
      841 ggagacccct gcccagggac caccgaccca ccaccgggag gtaagctggc cagcaactta
      901 tctgtgtctg tccgattgtc tagtgtctat gactgatttt atgcgcctgc gtcggtacta
      961 gttagctaac tagctctgta tctggcggac ccgtggtgga actgacgagt tcggaacacc
     1021 cggccgcaac cctgggagac gtcccaggga cttcgggggc cgtttttgtg gcccgacctg
     1081 agtccaaaaa tcccgatcgt tttggactct ttggtgcacc ccccttagag gagggatatg
     1141 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
     1201 cggtttggga ccgaagccgc gccgcgcgtc ttgtctgctg cagcatcgtt ctgtgttgtc
     1261 tctgtctgac tgtgtttctg tatttgtctg aaaatttggg cccgggccag actgttacca
     1321 ctcccttaag tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt
     1381 cggtagttgt caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta
     1441 acgtcggatg gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca
     1501 aggtcttttc acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg
     1561 aagccttggc ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc
     1621 ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc
     1681 gatcctccct ttatccagcc ctcactcctt ctctaggcgc ccccatatgg ccatatgaga
     1741 tcttatatgg ggcacccccg ccccttgtaa acttccctga ccctgacttg acaagagtta
     1801 ctaacagccc ctctctccaa gctcacttac aggctctcta cttagtccag cacgaagtct
     1861 ggagacctct ggcggcagcc taccaagaac aactggaccg accggtggta cctcaccctt
     1921 accgagtcgg cgacacagtg tgggtccgcc gacaccagac taagaaccta gaacctcgct
     1981 ggaaaggacc ttacacagtc ctgctgacca cccccaccgc cctcaaagta gacggcatcg
     2041 cagcttggat acacgccgcc cacgtgaagg ctgccgaccc cgggggtgga ccatcctcta
     2101 gactgccgga tcccagtgtg gtggtacggg aattcgccct tggctccgtt ccatccttct
     2161 gcacagggta tcgcctctct ccgtttggta catcccctcc tcccccacgc ccggactggg
     2221 gtggtagacg ccgcctccgc tcatcgcccc tccccatcgg tttccgcgcg aaaagccggg
     2281 gcgcctgcgc tgccgccgcc gcgtctgctg aagcctccga gatgccggcg cgtaccgccc
     2341 cagcccgggt gcccacactg gccgtcccgg ccatctcgct gcccgacgat gtccgcaggc
     2401 ggctcaaaga tttggaaaga gacagcttaa cagaaaagga atgtgtgaag gagaaattga
     2461 atctcttgca cgaatttctg caaacagaaa taaagaatca gttatgtgac ttggaaacca
     2521 aattacgtaa agaagaatta tccgaggagg gctacctggc taaagtcaaa tcccttttaa
     2581 ataaagattt gtccttggag aacggtgctc atgcttacaa ccgggaagtg aatggacgtc
     2641 tagaaaacgg gaaccaagca agaagtgaag cccgtagagt gggaatggca gatgccaaca
     2701 gcccccccaa acccctttcc aaacctcgca cgcccaggag gagcaagtcc gatggagagg
     2761 ctaagcgttc aagagaccct cctgcctcag cctcccaagt aactgggatt agagctgaac
     2821 cttcacctag ccccaggatt acaaggaaaa gcaccaggca aaccaccatc acatctcatt
     2881 ttgcaaaggg ccctgccaaa cggaaacctc aggaagagtc tgaaagagcc aaatcggatg
     2941 agtccatcaa ggaagaagac aaagaccagg atgagaagag acgtagagtt acatccagag
     3001 aacgagttgc tagaccgctt cctgcagaag aacctgaaag agcaaaatca ggaacgcgca
     3061 ctgaaaagga agaagaaaga gatgaaaaag aagaaaagag actccgaagt caaaccaaag
     3121 aaccaacacc caaacagaaa ctgaaggagg agccggacag agaagccagg gcaggcgtgc
     3181 aggctgacga ggacgaagat ggagacgaga aagatgagaa gaagcacaga agtcaaccca
     3241 aagatctagc tgccaaacgg aggcccgaag aaaaagaacc tgaaaaagta aatccacaga
     3301 tttctgatga aaaagacgag gatgaaaagg aggagaagag acgcaaaacg acccccaaag
     3361 aaccaacgga gaaaaaaatg gctcgcgcca aaacagtcat gaactccaag acccaccctc
     3421 ccaagtgcat tcagtgcggg cagtacctgg acgaccctga cctcaaatat gggcagcacc
     3481 caccagacgc ggtggatgag ccacagatgc tgacaaatga gaagctgtcc atctttgatg
     3541 ccaacgagtc tggctttgag agttatgagg cgcttcccca gcacaaactg acctgcttca
     3601 gtgtgtactg taagcacggt cacctgtgtc ccatcgacac cggcctcatc gagaagaata
     3661 tcgaactctt cttttctggt tcagcaaaac caatctatga tgatgacccg tctcttgaag
     3721 gtggtgttaa tggcaaaaat cttggcccca taaatgaatg gtggatcact ggctttgatg
     3781 gaggtgaaaa ggccctcatc ggcttcagca cctcatttgc cgaatacatt ctgatggatc
     3841 ccagtcccga gtatgcgccc atatttgggc tgatgcagga gaagatctac atcagcaaga
     3901 ttgtggtgga gttcctgcag agcaattccg actcgaccta tgaggacctg atcaacaaga
     3961 tcgagaccac ggttcctcct tctggcctca acttgaaccg cttcacagag gactccctcc
     4021 tgcgacacgc gcagtttgtg gtggagcagg tggagagtta tgacgaggcc ggggacagtg
     4081 atgagcagcc catcttcctg acgccctgca tgcgggacct gatcaagctg gctggggtca
     4141 cgctgggaca gaggcgagcc caggcgaggc ggcagaccat caggcattct accagggaga
     4201 aggacagggg acccacgaaa gccaccacca ccaagctggt ctaccagatc ttcgatactt
     4261 tcttcgcaga gcaaattgaa aaggatgaca gagaagacaa ggagaacgcc tttaagcgcc
     4321 ggcgatgtgg cgtctgtgag gtgtgtcagc agcctgagtg tgggaaatgt aaagcctgca
     4381 aggacatggt taaatttggt ggcagtggac ggagcaagca ggcttgccaa gagcggaggt
     4441 gtcccaatat ggccatgaag gaggcagatg acgatgagga agtcgatgat aacatcccag
     4501 agatgccgtc acccaaaaaa atgcaccagg ggaagaagaa gaaacagaac aagaatcgca
     4561 tctcttgggt cggagaagcc gtcaagactg atgggaagaa gagttactat aagaaggtgt
     4621 gcattgatgc ggaaaccctg gaagtggggg actgtgtctc tgttattcca gatgattcct
     4681 caaaaccgct gtatctagca agggtcacgg cgctgtggga ggacagcagc aacgggcaga
     4741 tgtttcacgc ccactggttc tgcgctggga cagacacagt cctcggggcc acgtcggacc
     4801 ctctggagct gttcttggtg gatgaatgtg aggacatgca gctttcatat atccacagca
     4861 aagtgaaagt catctacaaa gccccctccg aaaactgggc catggaggga ggcatggatc
     4921 ccgagtccct gctggagggg gacgacggga agacctactt ctaccagctg tggtatgatc
     4981 aagactacgc gagattcgag tcccctccaa aaacccagcc aacagaggac aacaagttca
     5041 aattctgtgt gagctgtgcc cgtctggctg agatgaggca aaaagaaatc cccagggtcc
     5101 tggagcagct cgaggacctg gatagccggg tcctctacta ctcagccacc aagaacggca
     5161 tcctgtaccg agttggtgat ggtgtgtacc tgccccctga ggccttcacg ttcaacatca
     5221 agctgtccag tcccgtgaaa cgcccacgga aggagcccgt ggatgaggac ctgtacccag
     5281 agcactaccg gaaatactcc gactacatca aaggcagcaa cctggatgcc cctgagccct
     5341 accgaattgg ccggatcaaa gagatcttct gtcccaagaa gagcaacggc aggcccaatg
     5401 agactgacat caaaatccgg gtcaacaagt tctacaggcc tgagaacacc cacaagtcca
     5461 ctccagcgag ctaccacgca gacatcaacc tgctctactg gagcgacgag gaggccgtgg
     5521 tggacttcaa ggctgtgcag ggccgctgca ccgtggagta tggggaggac ctgcccgagt
     5581 gcgtccaggt gtactccatg ggcggcccca accgcttcta cttcctcgag gcctataatg
     5641 caaagagcaa aagctttgaa gatcctccca accatgcccg tagccctgga aacaaaggga
     5701 agggcaaggg aaaagggaag ggcaagccca agtcccaagc ctgtgagccg agcgagccag
     5761 agatagagat caagctgccc aagctgcgga ccctggatgt gttttctggc tgcggggggt
     5821 tgtcggaggg attccaccaa gcaggcatct ctgacacgct gtgggccatc gagatgtggg
     5881 accctgcggc ccaggcgttc cggctgaaca accccggctc cacagtgttc acagaggact
     5941 gcaacatcct gctgaagctg gtcatggctg gggagaccac caactcccgc ggccagcggc
     6001 tgccccagaa gggagacgtg gagatgctgt gcggcgggcc gccctgccag ggcttcagcg
     6061 gcatgaaccg cttcaattcg cgcacctact ccaagttcaa aaactctctg gtggtttcct
     6121 tcctcagcta ctgcgactac taccggcccc ggttcttcct cctggagaat gtcaggaact
     6181 ttgtctcctt caagcgctcc atggtcctga agctcaccct ccgctgcctg gtccgcatgg
     6241 gctatcagtg caccttcggc gtgctgcagg ccggtcagta cggcgtggcc cagactagga
     6301 ggcgggccat catcctggcc gcggcccctg gagagaagct ccctctgttc ccggagccac
     6361 tgcacgtgtt tgctccccgg gcctgccagc tgagcgtggt ggtggatgac aagaagtttg
     6421 tgagcaacat aaccaggttg agctcgggtc ctttccggac catcacggtg cgagacacga
     6481 tgtccgacct gccggaggtg cggaatggag cctcggcact ggagatctcc tacaacgggg
     6541 agcctcagtc ctggttccag aggcagctcc ggggcgcaca gtaccagccc atcctcaggg
     6601 accacatctg taaggacatg agtgcattgg tggctgcccg catgcggcac atccccttgg
     6661 ccccagggtc agactggcgc gatctgccca acatcgaggt gcggctctca gacggcacca
     6721 tggccaggaa gctgcggtat acccaccatg acaggaagaa cggccgcagc agctctgggg
     6781 ccctccgtgg ggtctgctcc tgcgtggaag ccggcaaagc ctgcgacccc gcagccaggc
     6841 agttcaacac cctcatcccc tggtgcctgc cccacaccgg gaaccggcac aaccactggg
     6901 ctggcctcta tggaaggctc gagtgggacg gcttcttcag cacaaccgtc accaaccccg
     6961 agcccatggg caagcagggc cgcgtgctcc acccagagca gcaccgtgtg gtgagcgtgc
     7021 gggagtgtgc ccgctcccag ggcttccctg acacctaccg gctcttcggc aacatcctgg
     7081 acaagcaccg gcaggtgggc aatgccgtgc caccgcccct ggccaaagcc attggcttgg
     7141 agatcaagct ttgtatgttg gccaaagccc gagagagtgc ctcagctaaa ataaaggagg
     7201 aggaagctgc taaggactag ttctgccctc ccgtcacccc tgtttctggc accaggaatc
     7261 cccaacatgc actgatgttg tgtttttaac atgtcaatct gtccgttcac atgtgtggta
     7321 catggtgttt gtggccttgg ctgacataag aaacaaaggg cgaattccag ctgagcgccg
     7381 gtcgctacca ttaccagttg gtctggtgtc aaaaataata ataaccgggc aggccatgtc
     7441 tgcccgtatt tcgcgtaagg aaatccatta tgtactattt tcgagcggcc gccagcacag
     7501 tggtcgacga taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac
     7561 cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaaat
     7621 acataactga gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat
     7681 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
     7741 ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg
     7801 tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct
     7861 cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact
     7921 cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct
     7981 cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
     8041 tgactacccg tcagcggggg tctttcacat gcagcatgta tcaaaattaa tttggttttt
     8101 tttcttaagt atttacatta aatggccata gttgcattaa tgaatcggcc aacgcgcggg
     8161 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     8221 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     8281 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     8341 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     8401 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     8461 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     8521 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     8581 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     8641 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     8701 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     8761 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     8821 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     8881 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     8941 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     9001 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     9061 ccttttgcgg ccgaattaga tccagacatg ataagataca ttgatgagtt tggacaaacc
     9121 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     9181 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     9241 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     9301 ggtatggctg attatgatcc tctagagtcg gtgggcctcg ggggcgggtg cggggtcggc
     9361 ggggccgccc cgggtggctt cggtcggagc catggggtcg tgcgctcctt tcggtcgggc
     9421 gctgcgggtc gtggggcggg cgtcaggcac cgggcttgcg ggtcatgcac caggtgcgcg
     9481 gtccttcggg cacctcgacg tcggcggtga cggtgaagcc gagccgctcg tagaagggga
     9541 ggttgcgggg cgcggaggtc tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca
     9601 ctccggggag cacgacggcg ctgcccagac ccttgccctg gtggtcgggc gagacgccga
     9661 cggtggccag gaaccacgcg ggctccttgg gccggtgcgg cgccaggagg ccttccatct
     9721 gttgctgcgc ggccagccgg gaaccgctca actcggccat gcgcgggccg atctcggcga
     9781 acaccgcccc cgcttcgacg ctctccggcg tggtccagac cgccaccgcg gcgccgtcgt
     9841 ccgcgaccca caccttgccg atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct
     9901 cggtgacccg ctcgatgtgg cggtccgggt cgacggtgtg gcgcgtggcg gggtagtcgg
     9961 cgaacgcggc ggcgagggtg cgtacggccc gggggacgtc gtcgcgggtg gcgaggcgca
    10021 ccgtgggctt gtactcggtc atggaaggtc gtctccttgt gaggggtcag gggcgtgggt
    10081 caggggatgg tggcggcacc ggtcgtggcg gccgacctgc aggtcgaaag gcccggagat
    10141 gaggaagagg agaacagcgc ggcagacgtg cgcttttgaa gcgtgcagaa tgccgggcct
    10201 ccggaggacc ttcgggcgcc cgccccgccc ctgagcccgc ccctgagccc gcccccggac
    10261 ccaccccttc ccagcctctg agcccagaaa gcgaaggagc aaagctgcta ttggccgctg
    10321 ccccaaaggc ctacccgctt ccattgctca gcggtgctgt ccatctgcac gagactagtg
    10381 agacgtgcta cttccatttg tcacgtcctg cacgacgcga gctgcggggc gggggggaac
    10441 ttcctgacta ggggaggagt agaaggtggc gcgaaggggc caccaaagaa cggagccggt
    10501 tggcgcctac cggtggatgt ggaatgtgtg cgaggccaga ggccacttgt gtagcgccaa
    10561 gtgcccagcg gggctgctaa agcgcatgct ccagactgcc ttgggaaaag cgcctcccct
    10621 acccggtaga attaattctc atgtttgaca gcttatcatc gataagctga tcctcacagg
    10681 ccgcacccag cttttcttcc gttgccccag tagcatctct gtctggtgac cttgaagagg
    10741 aagaggaggg gtcccgagaa tccccatccc taccgtccag caaaaagggg gacgaggaat
    10801 ttgaggcctg gcttgaggct caggacgcaa atcttgagga tgttcagcgg gagttttccg
    10861 ggctgcgagt aattggtgat gaggacgagg atggttcgga ggatggggaa ttttcagacc
    10921 tggatctgtc tgacagcgac catgaagggg atgagggtgg gggggctgtt ggagggggca
    10981 ggagtctgca ctccctgtat tcactgagcg tcgtctaata aagatgtcta ttgatctctt
    11041 ttagtgtgaa tcatgtctga cgaggggcca ggtacaggac ctggaaatgg cctaggagag
    11101 aagggagaca catctggacc agaaggctcc ggcggcagtg gacctcaaag aagagggggt
    11161 gataaccatg gacgaggacg gggaagagga cgaggacgag gaggcggaag accaggagcc
    11221 ccgggcggct caggatcagg gccaagacat agagatggtg tccggagacc ccaaaaacgt
    11281 ccaagttgca ttggctgcaa agggacccac ggtggaacag gagcaggagc aggagcggga
    11341 ggggcaggag caggaggggc aggagcagga ggaggggcag gagcaggagg ggcaggagca
    11401 ggaggtggag gccggggtcg aggaggcagt ggaggccggg gtcgaggagg tagtggaggc
    11461 cggggtcgag gaggtagtgg aggccgccgg ggtagaggac gtgaaagagc caggggggga
    11521 agtcgtgaaa gagccagggg gagaggtcgt ggacgtggag aaaagaggcc caggagtccc
    11581 agtagtcagt catcatcatc cgggtctcca ccgcgcaggc cccctccagg tagaaggcca
    11641 tttttccacc ctgtagggga agccgattat tttgaatacc accaagaagg tggcccagat
    11701 ggtgagcctg acgtgccccc gggagcgata gagcagggcc ccgcagatga cccaggagaa
    11761 ggcccaagca ctggaccccg gggtcagggt gatggaggca ggcgcaaaaa aggagggtgg
    11821 tttggaaagc atcgtggtca aggaggttcc aacccgaaat ttgagaacat tgcagaaggt
    11881 ttaagagctc tcctggctag gagtcacgta gaaaggacta ccgacgaagg aacttgggtc
    11941 gccggtgtgt tcgtatatgg aggtagtaag acctcccttt acaacctaag gcgaggaact
    12001 gcccttgcta ttccacaatg tcgtcttaca ccattgagtc gtctcccctt tggaatggcc
    12061 cctggacccg gcccacaacc tggcccgcta agggagtcca ttgtctgtta tttcatggtc
    12121 tttttacaaa ctcatatatt tgctgaggtt ttgaaggatg cgattaagga ccttgttatg
    12181 acaaagcccg ctcctacctg caatatcagg gtgactgtgt gcagctttga cgatggagta
    12241 gatttgcctc cctggtttcc acctatggtg gaaggggctg ccgcggaggg tgatgacgga
    12301 gatgacggag atgaaggagg tgatggagat gagggtgagg aagggcagga gtgatgtaac
    12361 ttgttaggag acgccctcaa tcgtattaaa agccgtgtat tcccccgcac taaagaataa
    12421 atccccagta gacatcatgc gtgctgttgg tgtatttctg gccatctgtc ttgtcaccat
    12481 tttcgtcctc ccaacatggg gcaattgggc atacccatgt tgtcacgtca ctcagctccg
    12541 cgctcaacac cttctcgcgt tggaaaacat tagcgacatt tacctggtga gcaatcagac
    12601 atgcgacggc tttagcctgg cctccttaaa ttcacctaag aatgggagca accagcagga
    12661 aaaggacaag cagcgaaaat tcacgccccc ttgggaggtg gcggcatatg caaaggatag
    12721 cactcccact ctactactgg gtatcatatg ctgactgtat atgcatgagg atagcatatg
    12781 ctacccggat acagattagg atagcatata ctacccagat atagattagg atagcatatg
    12841 ctacccagat atagattagg atagcctatg ctacccagat ataaattagg atagcatata
    12901 ctacccagat atagattagg atagcatatg ctacccagat atagattagg atagcctatg
    12961 ctacccagat atagattagg atagcatatg ctacccagat atagattagg atagcatatg
    13021 ctatccagat atttgggtag tatatgctac ccagatataa attaggatag catatactac
    13081 cctaatctct attaggatag catatgctac ccggatacag attaggatag catatactac
    13141 ccagatatag attaggatag catatgctac ccagatatag attaggatag cctatgctac
    13201 ccagatataa attaggatag catatactac ccagatatag attaggatag catatgctac
    13261 ccagatatag attaggatag cctatgctac ccagatatag attaggatag catatgctat
    13321 ccagatattt gggtagtata tgctacccat ggcaacatta gcccaccgtg ctctcagcga
    13381 cctcgtgaat atgaggacca acaaccctgt gcttggcgct caggcgcaag tgtgtgtaat
    13441 ttgtcctcca gatcgcagca atcgcgcccc tatcttggcc cgcccaccta cttatgcagg
    13501 tattccccgg ggtgccatta gtggttttgt gggcaagtgg tttgaccgca gtggttagcg
    13561 gggttacaat cagccaagtt attacaccct tattttacag tccaaaaccg cagggcggcg
    13621 tgtgggggct gacgcgtgcc cccactccac aatttcaaaa aaaagagtgg ccacttgtct
    13681 ttgtttatgg gccccattgg cgtggagccc cgtttaattt tcgggggtgt tagagacaac
    13741 cagtggagtc cgctgctgtc ggcgtccact ctctttcccc ttgttacaaa tagagtgtaa
    13801 caacatggtt cacctgtctt ggtccctgcc tgggacacat cttaataacc ccagtatcat
    13861 attgcactag gattatgtgt tgcccatagc cataaattcg tgtgagatgg acatccagtc
    13921 tttacggctt gtccccaccc catggatttc tattgttaaa gatattcaga atgtttcatt
    13981 cctacactag tatttattgc ccaaggggtt tgtgagggtt atattggtgt catagcacaa
    14041 tgccaccact gaaccccccg tccaaatttt attctggggg cgtcacctga aaccttgttt
    14101 tcgagcacct cacatacacc ttactgttca caactcagca gttattctat tagctaaacg
    14161 aaggagaatg aagaagcagg cgaagattca ggagagttca ctgcccgctc cttgatcttc
    14221 agccactgcc cttgtgacta aaatggttca ctaccctcgt ggaatcctga ccccatgtaa
    14281 ataaaaccgt gacagctcat ggggtgggag atatcgctgt tccttaggac ccttttacta
    14341 accctaattc gatagcatat gcttcccgtt gggtaacata tgctattgaa ttagggttag
    14401 tctggatagt atatactact acccgggaag catatgctac ccgtttaggg ttaacaaggg
    14461 ggccttataa acactattgc taatgccctc ttgagggtcc gcttatcggt agctacacag
    14521 gcccctctga ttgacgttgg tgtagcctcc cgtagtcttc ctgggcccct gggaggtaca
    14581 tgtcccccag cattggtgta agagcttcag ccaagagtta cacataaagg caatgttgtg
    14641 ttgcagtcca cagactgcaa agtctgctcc aggatgaaag ccactcagtg ttggcaaatg
    14701 tgcacatcca tttataagga tgtcaactac agtcagagaa cccctttgtg tttggtcccc
    14761 ccccgtgtca catgtggaac agggcccagt tggcaagttg taccaaccaa ctgaagggat
    14821 tacatgcact gccccgcgaa gaaggggcag agatgtcgta gtcaggttta gttcgtccgg
    14881 ggcggggatc tggccgcaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    14941 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    15001 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    15061 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    15121 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    15181 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    15241 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    15301 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    15361 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    15421 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    15481 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    15541 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    15601 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    15661 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    15721 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    15781 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    15841 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    15901 cgcgcacatt tccccgaaaa gtgccaccag ctttgctctt aggagtttcc taatacatcc
    15961 caaactcaaa tatataaagc atttgacttg ttctatgccc tagggggcgg ggggaagcta
    16021 agccagcttt ttttaacatt taaaatgtta attccatttt aaatgcacag atgtttttat
    16081 ttcataaggg tt
//
