LOCUS       sequence-154451-         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Promoter1 with BCD1-gfp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Linshiz G, Stawski N, Goyal G, Bi C, Poust S, Sharma M, Mutalik V, 
            Keasling JD, Hillson NJ
  TITLE     PR-PR: cross-platform laboratory automation system.
  JOURNAL   ACS Synth Biol. 2014 Aug 15;3(8):515-24. doi: 10.1021/sb4001728. 
            Epub 2014 Jan 14.
  PUBMED    25126893
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001728";
            journalName: "ACS Synth Biol"; date: "2014-08-15- 15"; volume: "3"; 
            issue: "8"; pages: "515-24"
COMMENT     Sequence Label: pProm1_BCD1-GFP
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(1..708,709..711))
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELY"
     CDS             complement(1..711)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELY"
     primer_bind     complement(38..58)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     653..681
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gtagagctca tccatgccat gtgtaatccc agcagcagtt acaaactcaa gaaggaccat
       61 gtggtcacgc ttttcgttgg gatctttcga aaggacagat tgtgtcgaca ggtaatggtt
      121 gtctggtaaa aggacagggc catcgccaat tggagtattt tgttgataat ggtctgctag
      181 ttgaacggaa ccatcttcaa cgttgtggcg aattttgaag ttagctttga ttccattctt
      241 ttgtttgtct gccgtgatgt atacattgtg tgagttaaag ttgtactcga gtttgtgtcc
      301 aagaatgttt ccatcttctt taaaatcaat accctttaac tcgatacgat taacaagggt
      361 atcaccttca aacttgactt cagcacgcgt cttgtaggtc ccgtcatctt tgaaagatat
      421 agtgcgttcc tgtacataac cttcgggcat ggcactcttg aaaaagtcat gccgtttcat
      481 gtgatccgga taacgggaaa agcattgaac accataggtc agagtagtga caagtgttgg
      541 ccacggaaca ggtagttttc cagtagtgca aataaattta agggtgagtt ttccgtttgt
      601 agcatcacct tcaccctctc cacggacaga aaatttgtgc ccattaacat caccatctaa
      661 ttcaacaaga attgggacaa ctccagtgaa aagttcttct cctttgctca ttagaaagtc
      721 tcctgtgcat gattaagatg tttcagtacg aaaattgctt tcattgttga tctccttttt
      781 aagtgaactt gggccctcca cacattatac ctataggtta gactttaagt caatactctt
      841 tttgttatgc agcaacgact catagaaagc acgcagctga tagggtcgac gtggtgtttg
      901 ctgtgcgttg gacgtgtgga ttgggatgaa aataccccca taaaaaaagg cgccgaa
//
