LOCUS       sequence-154452-        8367 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  a modified episomal (EBNA1/OriP) vector expressing human BCL2L1 
            (BCL-xL) gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8367)
  AUTHORS   Chou BK, Gu H, Gao Y, Dowey SN, Wang Y, Shi J, Li Y, Ye Z, Cheng T, 
            Cheng L
  TITLE     A Facile Method to Establish Human Induced Pluripotent Stem Cells 
            From Adult Blood Cells Under Feeder-Free and Xeno-Free Culture 
            Conditions: A Clinically Compliant Approach.
  JOURNAL   Stem Cells Transl Med. 2015 Mar 5. pii: sctm.2014-0214.
  PUBMED    25742692
REFERENCE   2  (bases 1 to 8367)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells 
            Transl Med. 2015 Mar 5. pii: sctm.2014-0214."
COMMENT     Sequence Label: MBX
FEATURES             Location/Qualifiers
     source          1..8367
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(9..27)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        95..199
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(200..268,269..1060)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(418..437)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1231..1819
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1720..1739
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2002..2409
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     primer_bind     complement(3194..3214)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3612..3623)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3720..3854
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3744..3763)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3798..3817
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3997..4019)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4582..6371
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
       61 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
      121 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      181 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
      241 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
      301 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
      361 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
      421 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
      481 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
      541 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
      601 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
      661 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
      721 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg
      781 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
      841 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
      901 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
      961 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     1021 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     1081 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     1141 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1201 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1261 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1321 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     1381 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1441 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1501 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1561 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1621 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1681 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     1741 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     1801 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     1861 ctggccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa
     1921 cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc
     1981 tcgcgtcgcg agctagctgc agtaacgcca ttttgcaagg catggaaaaa taccaaacca
     2041 agaatagaga agttcagatc aagggcgggt acatgaaaat agctaacgtt gggccaaaca
     2101 ggatatctgc ggtgagcagt ttcggccccg gcccggggcc aagaacagat ggtcaccgca
     2161 gtttcggccc cggcccgagg ccaagaacag atggtcccca gatatggccc aaccctcagc
     2221 agtttcttaa gacccatcag atgtttccag gctcccccaa ggacctgaaa tgaccctgcg
     2281 ccttatttga attaaccaat cagcctgctt ctcgcttctg ttcgcgcgct tctgcttccc
     2341 gagctctata aaagagctca caacccctca ctcggcgcgc cagtcctccg acagactgag
     2401 tcgcccgggt accgcgatcg ccatgtctca gagcaaccgg gagctggtgg ttgactttct
     2461 ctcctacaag ctttcccaga aaggatacag ctggagtcag tttagtgatg tggaagagaa
     2521 caggactgag gccccagaag ggactgaatc ggagatggag acccccagtg ccatcaatgg
     2581 caacccatcc tggcacctgg cagacagccc cgcggtgaat ggagccactg gccacagcag
     2641 cagtttggat gcccgggagg tgatccccat ggcagcagta aagcaagcgc tgagggaggc
     2701 aggcgacgag tttgaactgc ggtaccggcg ggcattcagt gacctgacat cccagctcca
     2761 catcacccca gggacagcat atcagagctt tgaacaggta gtgaatgaac tcttccggga
     2821 tggggtaaac tggggtcgca ttgtggcctt tttctccttc ggcggggcac tgtgcgtgga
     2881 aagcgtagac aaggagatgc aggtattggt gagtcggatc gcagcttgga tggccactta
     2941 cctgaatgac cacctagagc cttggatcca ggagaacggc ggctgggata cttttgtgga
     3001 actctatggg aacaatgcag cagccgagag ccgaaagggc caggaacgct tcaaccgctg
     3061 gttcctgacg ggcatgactg tggccggcgt ggttctgctg ggctcactct tcagtcggaa
     3121 atgagtttaa acgcgtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     3181 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     3241 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     3301 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     3361 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     3421 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     3481 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     3541 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     3601 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     3661 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggcgg
     3721 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     3781 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     3841 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     3901 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
     3961 cggctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     4021 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     4081 agcgggtgtt ggcgggtgtc ggggcgcagc catgaggtcg actctactag aggatcgatc
     4141 cccgccccgg acgaactaaa cctgactacg acatctctgc cccttcttcg cggggcagtg
     4201 catgtaatcc cttcagttgg ttggtacaac ttgccaactg ggccctgttc cacatgtgac
     4261 acgggggggg accaaacaca aaggggttct ctgactgtag ttgacatcct tataaatgga
     4321 tgtgcacatt tgccaacact gagtggcttt catcctggag cagactttgc agtctgtgga
     4381 ctgcaacaca acattgcctt tatgtgtaac tcttggctga agctcttaca ccaatgctgg
     4441 gggacatgta cctcccaggg gcccaggaag actacgggag gctacaccaa cgtcaatcag
     4501 aggggcctgt gtagctaccg ataagcggac cctcaagagg gcattagcaa tagtgtttat
     4561 aaggccccct tgttaaccct aaacgggtag catatgcttc ccgggtagta gtatatacta
     4621 tccagactaa ccctaattca atagcatatg ttacccaacg ggaagcatat gctatcgaat
     4681 tagggttagt aaaagggtcc taaggaacag cgatatctcc caccccatga gctgtcacgg
     4741 ttttatttac atggggtcag gattccacga gggtagtgaa ccattttagt cacaagggca
     4801 gtggctgaag atcaaggagc gggcagtgaa ctctcctgaa tcttcgcctg cttcttcatt
     4861 ctccttcgtt tagctaatag aataactgct gagttgtgaa cagtaaggtg tatgtgaggt
     4921 gctcgaaaac aaggtttcag gtgacgcccc cagaataaaa tttggacggg gggttcagtg
     4981 gtggcattgt gctatgacac caatataacc ctcacaaacc ccttgggcaa taaatactag
     5041 tgtaggaatg aaacattctg aatatcttta acaatagaaa tccatggggt ggggacaagc
     5101 cgtaaagact ggatgtccat ctcacacgaa tttatggcta tgggcaacac ataatcctag
     5161 tgcaatatga tactggggtt attaagatgt gtcccaggca gggaccaaga caggtgaacc
     5221 atgttgttac actctatttg taacaagggg aaagagagtg gacgccgaca gcagcggact
     5281 ccactggttg tctctaacac ccccgaaaat taaacggggc tccacgccaa tggggcccat
     5341 aaacaaagac aagtggccac tctttttttt gaaattgtgg agtgggggca cgcgtcagcc
     5401 cccacacgcc gccctgcggt tttggactgt aaaataaggg tgtaataact tggctgattg
     5461 taaccccgct aaccactgcg gtcaaaccac ttgcccacaa aaccactaat ggcaccccgg
     5521 ggaatacctg cataagtagg tgggcgggcc aagatagggg cgcgattgct gcgatctgga
     5581 ggacaaatta cacacacttg cgcctgagcg ccaagcacag ggttgttggt cctcatattc
     5641 acgaggtcgc tgagagcacg gtgggctaat gttgccatgg gtagcatata ctacccaaat
     5701 atctggatag catatgctat cctaatctat atctgggtag cataggctat cctaatctat
     5761 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatttat
     5821 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     5881 atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat cctaatagag
     5941 attagggtag tatatgctat cctaatttat atctgggtag catatactac ccaaatatct
     6001 ggatagcata tgctatccta atctatatct gggtagcata tgctatccta atctatatct
     6061 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
     6121 gggtagtata tgctatccta atttatatct gggtagcata ggctatccta atctatatct
     6181 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc
     6241 gggtagcata tgctatcctc atgcatatac agtcagcata tgatacccag tagtagagtg
     6301 ggagtgctat cctttgcata tgccgccacc tcccaagggg gcgtgaattt tcgctgcttg
     6361 tccttttcct gctggttgct cccattctta ggtgaattta aggaggccag gctaaagccg
     6421 tcgcatgtct gattgctcac caggtaaatg tcgctaatgt tttccaacgc gagaaggtgt
     6481 tgagcgcgga gctgagtgac gtgacaacat gggtatgccc aattgcccca tgttgggagg
     6541 acgaaaatgg tgacaagaca gatggccaga aatacaccaa cagcacgcat gatgtctact
     6601 ggggatttat tctttagtgc gggggaatac acggctttta atacgattga gggcgtctcc
     6661 taacaagtta catcactcct gcccttcctc accctcatct ccatcacctc cttcatctcc
     6721 gtcatctccg tcatcaccct ccgcggcagc cccttccacc ataggtggaa accagggagg
     6781 caaatctact ccatcgtcaa agctgcacac agtcaccctg atattgcagg taggagcggg
     6841 ctttgtcata acaaggtcct taatcgcatc cttcaaaacc tcagcaaata tatgagtttg
     6901 taaaaagacc atgaaataac agacaatgga ctcccttagc gggccaggtt gtgggccggg
     6961 tccaggggcc attccaaagg ggagacgact caatggtgta agacgacatt gtggaatagc
     7021 aagggcagtt cctcgcctta ggttgtaaag ggaggtctta ctacctccat atacgaacac
     7081 accggcgacc caagttcctt cgtcggtagt cctttctacg tgactcctag ccaggagagc
     7141 tcttaaacct tctgcaatgt tctcaaattt cgggttggaa cctccttgac cacgatgctt
     7201 tccaaaccac cctccttttt tgcgcctgcc tccatcaccc tgaccccggg gtccagtgct
     7261 tgggccttct cctgggtcat ctgcggggcc ctgctctatc gctcccgggg gcacgtcagg
     7321 ctcaccatct gggccacctt cttggtggta ttcaaaataa tcggcttccc ctacagggtg
     7381 gaaaaatggc cttctacctg gagggggcct gcgcggtgga gacccggatg atgatgactg
     7441 actactggga ctcctgggcc tcttttctcc acgtccacga cctctccccc tggctctttc
     7501 acgacttccc cccctggctc tttcacgtcc tctaccccgg cggcctccac tacctcctcg
     7561 accccggcct ccactacctc ctcgaccccg gcctccactg cctcctcgac cccggcctcc
     7621 acctcctgct cctgcccctc ctgctcctgc ccctcccgct cctgctcctg ctcctgttcc
     7681 accgtgggtc cctttgcagc caatgcaact tggacgtttt tggggtctcc ggacaccatc
     7741 tctatgtctt ggccctgatc ctgagccgcc cggggctcct ggtcttccgc ctcctcgtcc
     7801 tcgtcctctt ccccgtcctc gtccatggtt atcaccccct cttctttgag gtccactgcc
     7861 gccggagcct tctggtccag atgtgtctcc cttctctcct aggccatttc caggtcctgt
     7921 acctggcccc tcgtcagaca tgattcacac taaaagagat caatagacat ctttattaga
     7981 cgacgctcag tgaatacagg gagtgcagac tcctgccccc tccaacagcc cccccaccct
     8041 catccccttc atggtcgctg tcagacagat ccaggtctga aaattcccca tcctccgaac
     8101 catcctcgtc ctcatcacca attactcgca gcccggaaaa ctcccgctga acatcctcaa
     8161 gatttgcgtc ctgagcctca agccaggcct caaattcctc gtcccccttt ttgctggacg
     8221 gtagggatgg ggattctcgg gacccctcct cttcctcttc aaggtcacca gacagagatg
     8281 ctactggggc aacggaagaa aagctgggtg cggcctgtga ggatcagctt atcgatgata
     8341 agctgtcaaa catgagaatt cttgaag
//
