LOCUS       sequence-154455-         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Promoter1 with BCD20-gfp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Linshiz G, Stawski N, Goyal G, Bi C, Poust S, Sharma M, Mutalik V, 
            Keasling JD, Hillson NJ
  TITLE     PR-PR: cross-platform laboratory automation system.
  JOURNAL   ACS Synth Biol. 2014 Aug 15;3(8):515-24. doi: 10.1021/sb4001728. 
            Epub 2014 Jan 14.
  PUBMED    25126893
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001728";
            journalName: "ACS Synth Biol"; date: "2014-08-15- 15"; volume: "3"; 
            issue: "8"; pages: "515-24"
COMMENT     Sequence Label: pProm1_BCD20-GFP
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(414..442)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 agaaagccat ccagtttact ttgcagggct tcccaacctt accagagggc gccccagctg
       61 gcaattccga cgtcgaattc aaaagatctt aagtaagtaa gagtatacgt atatcggcta
      121 ataacgtatt aaggcgcttc ggcgcctttt tttatggggg tattttcatc ccaatccaca
      181 cgtccaacgc acagcaaaca ccacgtcgac cctatcagct gcgtgctttc tatgagtcgt
      241 tgctgcataa caaaaagagt attgacttaa agtctaacct ataggtataa tgtgtggagg
      301 gcccaagttc acttaaaaag gagatcaaca atgaaagcaa ttttcgtact gaaacatctt
      361 aatcatgctg aggaaagttt ctaatgagca aaggagaaga acttttcact ggagttgtcc
      421 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc cgtggagagg
      481 gtgaaggtga tgctacaaac ggaaaactca cccttaaatt tatttgcact actggaaaac
      541 tacctgttcc gtggccaaca cttgtcacta ctctgaccta tggtgttcaa tgcttttccc
      601 gttatccgga tcacatgaaa cggcatgact ttttcaagag tgccatgccc gaaggttatg
      661 tacaggaacg cactatatct ttcaaagatg acgggaccta caagacgcgt gctgaagtca
      721 agtttgaagg tgataccctt gttaatcgta tcgagttaaa gggtattgat tttaaagaag
      781 atggaaacat tcttggacac aaactcgagt acaactttaa ctcacacaat gtatacatca
      841 cggcagacaa acaaaagaat ggaatcaaag ctaacttcaa aattcgccac aacgttgaag
      901 atggttccgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg
      961 tccttttacc agacaaccat tacctg
//
