LOCUS       sequence-154456-         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the AAVS1 T2 gRNA in combination with FLAGless 
            eSpCas9(1.1) to target the AAVS1 ""safe harbor"" locus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Dalvai M, Loehr J, Jacquet K, Huard CC, Roques C, Herst P, Cote J, 
            Doyon Y
  TITLE     A Scalable Genome-Editing-Based Approach for Mapping Multiprotein 
            Complexes in Human Cells.
  JOURNAL   Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 
            10.1016/j.celrep.2015.09.009.
  PUBMED    26456817
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 
            10.1016/j.celrep.2015.09.009."
COMMENT     Sequence Label: eSpCas9(1.1)_No_FLAG_AAVS1_T2
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttctttccat gatggtgatc cccagcagct ctttcacact cttcagtttc ttggacttgc
       61 ccttttccac tttggccacc accagcacag aataggccac ggtggggctg tcgaagccgc
      121 cgtacttctt agggtcccag tccttctttc tggcgatcag cttatcgctg ttcctcttgg
      181 gcaggataga ctctttgctg aagccgcctg tctgcacctc ggtctttttc acgatattca
      241 cttggggcat gctcagcact ttccgcacgg tggcaaaatc ccggccctta tcccacacga
      301 tctccccggt ttcgccgttt gtctcgatca gaggcgcctt ccggatctcg ccgttggcca
      361 gggtaatctc ggtcttgaaa aagttcatga tgttgctgta gaagaagtac ttggcggtag
      421 ccttgccgat ttcctgctcg ctcttggcga tcatcttccg cacgtcgtac accttgtagt
      481 cgccgtacac gaactcgctt tccagcgcag ggtacttttt gatcagggcg gttcccacga
      541 cggcgttcag gtaggcgtcg tgggcgtggt ggtagttgtt gatctcgcgc actttgtaaa
      601 actggaaatc cttccggaaa tcggacacca gcttggactt cagggtgatc actttcactt
      661 cccggatcag cttgtcattc tcgtcgtact tagtgttcat ccgggagtcc aggatctgtg
      721 ccacgtgctt tgtgatctgc cgggtttcca ccagctgtct cttgatgaag ccggccttat
      781 ccagttcgct caggccgcct ctctcggcct tggtcagatt gtcgaacttt ctctgggtaa
      841 tcagcttggc gttcagcagc tgccgccagt agttcttcat cttcttcacg acctcttcgg
      901 agggcacgtt gtcgctcttg ccccggttct tgtcgcttct ggtcagcacc ttgttgtcga
      961 tggagtcgtc ggcca
//
