LOCUS       sequence-154457-        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Promoter1 with BCD20-gfp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Linshiz G, Stawski N, Goyal G, Bi C, Poust S, Sharma M, Mutalik V, 
            Keasling JD, Hillson NJ
  TITLE     PR-PR: cross-platform laboratory automation system.
  JOURNAL   ACS Synth Biol. 2014 Aug 15;3(8):515-24. doi: 10.1021/sb4001728. 
            Epub 2014 Jan 14.
  PUBMED    25126893
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001728";
            journalName: "ACS Synth Biol"; date: "2014-08-15- 15"; volume: "3"; 
            issue: "8"; pages: "515-24"
COMMENT     Sequence Label: pProm1_BCD20-GFP
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(3..716,717..719))
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(6..719)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(46..66)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     661..689
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ccttatttgt agagctcatc catgccatgt gtaatcccag cagcagttac aaactcaaga
       61 aggaccatgt ggtcacgctt ttcgttggga tctttcgaaa ggacagattg tgtcgacagg
      121 taatggttgt ctggtaaaag gacagggcca tcgccaattg gagtattttg ttgataatgg
      181 tctgctagtt gaacggaacc atcttcaacg ttgtggcgaa ttttgaagtt agctttgatt
      241 ccattctttt gtttgtctgc cgtgatgtat acattgtgtg agttaaagtt gtactcgagt
      301 ttgtgtccaa gaatgtttcc atcttcttta aaatcaatac cctttaactc gatacgatta
      361 acaagggtat caccttcaaa cttgacttca gcacgcgtct tgtaggtccc gtcatctttg
      421 aaagatatag tgcgttcctg tacataacct tcgggcatgg cactcttgaa aaagtcatgc
      481 cgtttcatgt gatccggata acgggaaaag cattgaacac cataggtcag agtagtgaca
      541 agtgttggcc acggaacagg tagttttcca gtagtgcaaa taaatttaag ggtgagtttt
      601 ccgtttgtag catcaccttc accctctcca cggacagaaa atttgtgccc attaacatca
      661 ccatctaatt caacaagaat tgggacaact ccagtgaaaa gttcttctcc tttgctcatt
      721 agaaactttc ctcagcatga ttaagatgtt tcagtacgaa aattgctttc attgttgatc
      781 tcctttttaa gtgaacttgg gccctccaca cattatacct ataggttaga ctttaagtca
      841 atactctttt tgttatgcag caacgactca tagaaagcac gcagctgata gggtcgacgt
      901 ggtgtttgct gtgcgttgga cgtgtggatt gggatgaaaa tacccccata aaaaaaggcg
      961 ccgaagcgcc ttaatacgtt attagccgat atacgtatac tcttacttac ttaagatctt
     1021 ttgaattc
//
