LOCUS       sequence-154513-         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Suicide vector for gene replacement.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Quandt J, Hynes MF
  TITLE     Versatile suicide vectors which allow direct selection for gene 
            replacement in gram-negative bacteria.
  JOURNAL   Gene. 1993 May 15;127(1):15-21.
  PUBMED    8486283
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "1993-05-15"; volume: "127(1)"; pages: "15-21"
COMMENT     Sequence Label: pJQ200SK
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(37..53)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(37..53)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(90..110)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(90..108)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(129..145)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(129..145)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(142..164)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    153..169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(177..183,184..201,202..207))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    222..243
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(502..519)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(716..735)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcgataagct tgatatcgaa ttcctgcagc ccgggggatc cactagttct agagcggccg
       61 ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg
      121 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
      181 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
      241 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccaggggaat
      301 taattctgtc cctcctgttc agctactgac ggggtggtgc gtaacggcaa aagcaccgcc
      361 ggacatcagc gctagcggag tgtatactgg cttactatgt tggcactgat gagggtgtca
      421 gtgaagtgct tcatgtggca ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg
      481 atacaggata tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
      541 gcgagcggaa atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact
      601 taacagggaa gtgagagggc cgcggcaaag ccgtttttcc ataggctccg cccccctgac
      661 aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga
      721 taccaggcgt ttccccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt
      781 acc
//
