LOCUS       sequence-154519-         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSB3K3 carrying 
            araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-30hrpR-30hrpS-t-hrpL-30gfp-t.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Wang B, Barahona M, Buck M
  TITLE     Engineering modular and tunable genetic amplifiers for scaling 
            transcriptional signals in cascaded gene networks.
  JOURNAL   Nucleic Acids Res. 2014 Aug;42(14):9484-92. doi: 10.1093/nar/gku593.
            Epub 2014 Jul 16.
  PUBMED    25030903
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku593"; journalName: "Nucleic Acids Res"; date: 
            "2014-08"; volume: "42"; issue: "14"; pages: "9484-92"
COMMENT     Sequence Label: pBW301ParsR-Amp30T
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..53
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             192..203
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      431..502
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      518..545
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        560..594
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
ORIGIN
        1 gctttttatc gcaactctct actgtttctc cattactaga gaaagaggag aaatactaga
       61 tgattgaggt aacggaaaag tcggcatttt acgctcaggt tgccgcccag agcccagcag
      121 tatggccagt ggccaatggc gtcgcgtttg tcagtcggcg cgaacaccat gattggggga
      181 tcgccttgca catagaaggc cgcgcgttgc gtcccgatca actgcgtgat gcactgcaac
      241 gacgttttat ggagtcagag cgtttcaatc actacttcct gtttctggac gtacgacgcg
      301 actttgtggt gtggcacgcg gtcaacgaaa aaccgggttc ctacgccagc ctggacgaca
      361 tccgccggca tgagttgatg ctggcagggc tggaccattt gagcgaggaa atgcactaat
      421 aaccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
      481 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
      541 ctgcgtttat atactagagt ttacagctag ctcagtcctt ggtattatgc tagctcctag
      601 ggtcacacag gaaagtacca tatgtcattt ctgttaccca tccaattgtt caaaattctt
      661 gctgatgaaa cccgtctggg catcgtttta ctgctcagcg aactgggaga gttatgcgtc
      721 tgcgatctct gcactgctct cgaccagtcg cagcccaaga tctcccgcca cctggcattg
      781 ctgcgtg
//
