LOCUS       sequence-154521-         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSB3K3 carrying 
            araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-30hrpR-30hrpS-t-hrpL-30gfp-t.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Wang B, Barahona M, Buck M
  TITLE     Engineering modular and tunable genetic amplifiers for scaling 
            transcriptional signals in cascaded gene networks.
  JOURNAL   Nucleic Acids Res. 2014 Aug;42(14):9484-92. doi: 10.1093/nar/gku593.
            Epub 2014 Jul 16.
  PUBMED    25030903
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku593"; journalName: "Nucleic Acids Res"; date: 
            "2014-08"; volume: "42"; issue: "14"; pages: "9484-92"
COMMENT     Sequence Label: pBW301ParsR-Amp30T
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..21
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(25..68)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    71..92
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg
       61 atgatttctg gaattcgcgg ccgcttctag agttatgaca acttgacggc tacatcattc
      121 actttttctt cacaaccggc acggaactcg ctcgggctgg ccccggtgca ttttttaaat
      181 acccgcgaga aatagagttg atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc
      241 atccgggtgg tgctcaaaag cagcttcgcc tggctgatac gttggtcctc gcgccagctt
      301 aagacgctaa tccctaactg ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa
      361 acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac
      421 tgacaagcct cgcgtacccg attatccatc ggtggatgga gcgactcgtt aatcgcttcc
      481 atgcgccgca gtaacaattg ctcaagcaga tttatcgcca gcagctccga atagcgccct
      541 tccccttgcc cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct
      601 tcatccgggc gaaagaaccc cgtattggca aatattgacg gccagttaag ccattcatgc
      661 cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc attcgcgagc ctccggatga
      721 cgaccgtagt gatgaatctc tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca
      781 aattctcgtc cctgattttt caccaccccc tgaccgcgaa tggtgagatt gagaatataa
      841 cctttcattc ccagcgggtc ggtcgataaa aaaatcgaga taaccgttgg cctcaatcgg
      901 cgttaaaccc gccaccagat gggcattaaa c
//
