LOCUS       sequence-154522-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cosmid version of suicide vector pJQ200SK. Suicide vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Quandt J, Hynes MF
  TITLE     Versatile suicide vectors which allow direct selection for gene 
            replacement in gram-negative bacteria.
  JOURNAL   Gene. 1993 May 15;127(1):15-21.
  PUBMED    8486283
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "1993-05-15"; volume: "127(1)"; pages: "15-21"
COMMENT     Sequence Label: pJQ210SK
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(47..63)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(47..63)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(100..120)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(100..118)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(139..155)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(139..155)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(152..174)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    163..179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(187..193,194..211,212..217))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    232..253
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(512..529)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(726..745)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtcgacggta tcgataagct tgatatcgaa ttcctgcagc ccgggggatc cactagttct
       61 agagcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattgc
      121 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
      181 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
      241 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
      301 ccaggggaat taattctgtc cctcctgttc agctactgac ggggtggtgc gtaacggcaa
      361 aagcaccgcc ggacatcagc gctagcggag tgtatactgg cttactatgt tggcactgat
      421 gagggtgtca gtgaagtgct tcatgtggca ggagaaaaaa ggctgcaccg gtgcgtcagc
      481 agaatatgtg atacaggata tattccgctt cctcgctcac tgactcgcta cgctcggtcg
      541 ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg gaagatgcca
      601 ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgtttttcc ataggctccg
      661 cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa acccgacagg
      721 actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc c
//
