LOCUS       sequence-154523-         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: PRDM15_pCSdest
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    109..133
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(610..621)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
       61 agctacttgt tctttttgca ggatcccatc gattcgaatt caaggatcac aagtttgtac
      121 aaaaaagcag gcaccatggc tgaagatggg agcgaagaga tcatgttcat ctggtgtgaa
      181 gactgcagcc agtaccacga ctccgaatgt cccgagctgg gcccagtggt catggtcaaa
      241 gactcctttg tgttaagcag ggcaaggtca tcccttcctc ccaacttgga gatcagacga
      301 ctggaagatg gagccgaggg ggtgttcgcc atcactcagc tcgtcaagcg gacacagttc
      361 ggtccctttg agtccaggag ggtcgccaaa tgggaaaagg agtctgcatt tcccctgaag
      421 gtgttccaga aggacgggca ccccgtgtgc ttcgacacct ccaacgagga tgactgcaac
      481 tggatgatgc tggtgcggcc agcggcggag gccgagcacc agaacctgac ggcctaccag
      541 cacggcagcg acgtgtactt caccacctcc agagacatcc ccccgggtac cgagctgcgc
      601 gtgtggtatg cggccttcta tgccaagaag atggacaagc ccatgctgaa gcaggccggc
      661 tctggcgtcc acgctgcagg caccccagaa aacagcgccc ccgtggagtc ggaacccagc
      721 cagtgggcgt gtaaagtgtg ttctgccacc ttcctggagc tgcagctcct caatgaacat
      781 ctgttgggcc acttagaaca agccaaaagc cttcctccag gcagccaa
//
