LOCUS       sequence-154528-         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically 
            selected.Modified from BBa_J23100. [A:36INS-J23100:B].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 142 to 176)
  AUTHORS   Carr SB, Beal J, Densmore DM
  TITLE     Reducing DNA context dependence in bacterial promoters.
  JOURNAL   PLoS One. 2017 Apr 19;12(4):e0176013. doi: 
            10.1371/journal.pone.0176013. eCollection 2017.
  PUBMED    28422998
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2017-04-19"; volume: "12(4):e0176013. doi"; pages: " 
            10.1371/journal.pone.0176013. eCollection 2017"
COMMENT     Sequence Label: 36INS-J23100_AB
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     87..104
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    110..130
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     promoter        complement(142..176)
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     misc_feature    231..252
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      255..298
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(302..320)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        388..492
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(711..730)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
       61 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcc tgcagcggcc
      121 gctactagta ggtctctagt agctagcact gtacctagga ctgagctagc cgtcaaatct
      181 accttccgta agctgaaaat gaatactgca ggctcctgag acccactagt ctctagaagc
      241 ggccgcgaat tccagaaatc atccttagcg aaagctaagg atttttttta tctgaaattc
      301 tgcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
      361 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
      421 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
      481 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
      541 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
      601 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
      661 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
      721 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
      781 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
      841 gtaagagaat tatgcagtgc tgc
//
