LOCUS       sequence-154534-         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically 
            selected. Modified from BBa_J23100. [F:36INS-J23100:B].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 145 to 179)
  AUTHORS   Carr SB, Beal J, Densmore DM
  TITLE     Reducing DNA context dependence in bacterial promoters.
  JOURNAL   PLoS One. 2017 Apr 19;12(4):e0176013. doi: 
            10.1371/journal.pone.0176013. eCollection 2017.
  PUBMED    28422998
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2017-04-19"; volume: "12(4):e0176013. doi"; pages: " 
            10.1371/journal.pone.0176013. eCollection 2017"
COMMENT     Sequence Label: 36INS-J23100_FB
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..19
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(23..66)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    69..90
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        145..179
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     misc_feature    191..211
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(217..234)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(468..487)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat
       61 gatttctgga attcgcggcc gcttctagag actagtgggt ctcacgctcc tgcagtattc
      121 attttcagct tacggaaggt agatttgacg gctagctcag tcctaggtac agtgctagct
      181 actagagacc tactagtagc ggccgctgca ggcttcctcg ctcactgact cgctgcgctc
      241 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      301 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
      361 ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca
      421 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      481 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      541 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
      601 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
      661 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
      721 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
      781 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
      841 tatctgcgct ctgctga
//
